Adrenergic innervation of corticotropin releasing factor (CRF) - synthesizing neurons in the hypothalamic paraventricular nucleus of the rat - A combined light and electron microscopic immunocytochemical study

Zs Liposits, C. Phelix, W. K. Paull

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Corticotropin releasing factor (CRF), a neuropeptide synthesized in the parvocellular subnuclei of the hypothalamic paraventricular nucleus (PVN), takes part in the regulation of different stress evoked responses of the organism. In order to elucidate the role of the central adrenergic system in the regulation of these CRF-synthesizing neurons, a novel ultrastructural immunocytochemical dual localization technique was utilized. Phenylethanolamine-N-methyltransferase (PNMT), a specific enzyme marker for the central adrenaline system, and CRF-immunoreactive elements were simultaneously visualized in hypothalamic sections. PNMT-immunoreactive axon terminals established synaptic connections with somata, dendrites and spinous structures of CRF-producing neurons. This morphological finding indicates that the central adrenergic system directly influences CRF-synthesizing neurons in the PVN and provides basis for a more definitive pharmacological manipulation of this system.

Original languageEnglish
Pages (from-to)201-205
Number of pages5
Issue number3
Publication statusPublished - May 1 1986


ASJC Scopus subject areas

  • Anatomy
  • Agricultural and Biological Sciences(all)

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