Adhesion force measurements on functionalized microbeads: An in-depth comparison of computer controlled micropipette and fluidic force microscopy

Tamás Gerecsei, István Erdődi, Beatrix Peter, Csaba Hős, Sándor Kurunczi, I. Derényi, B. Szabó, R. Horváth

Research output: Contribution to journalArticle

Abstract

Characterization of the binding of functionalized microparticles to surfaces with a specific chemistry sheds light on molecular scale interactions. Polymer or protein adsorption are often monitored by colloid particle deposition. Force measurements on microbeads by atomic force microscopy (AFM) or optical tweezers are standard methods in molecular biophysics, but typically have low throughput. Washing and centrifuge assays with (bio)chemically decorated microbeads provide better statistics, but only qualitative results without a calibrated binding force or energy value. In the present work we demonstrate that a computer controlled micropipette (CCMP) is a straightforward and high-throughput alternative to quantify the surface adhesion of functionalized microparticles. However, being an indirect force measurement technique, its in-depth comparison with a direct force measurement is a prerequisite of applications requiring calibrated adhesion force values. To this end, we attached polystyrene microbeads to a solid support by the avidin-biotin linkage. We measured the adhesion strength of the microbeads with both a specialized robotic fluid force microscope (FluidFM BOT) and CCMP. Furthermore, the bead-support contact zone was directly characterized on an optical waveguide biosensor to determine the density of avidin molecules. Distribution of the detachment force recorded on ∼50 individual beads by FluidFM BOT was compared to the adhesion distribution obtained from CCMP measurements on hundreds of individual beads. We found that both methods provide unimodal histograms. We conclude that FluidFM BOT can directly measure the detachment force curve of 50 microbeads in 150 min. CCMP can provide calibrated binding/adhesion force values of 120 microbeads in an hour.

Original languageEnglish
Pages (from-to)245-253
Number of pages9
JournalJournal of Colloid And Interface Science
Volume555
DOIs
Publication statusPublished - Nov 1 2019

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Force measurement
Fluidics
Microscopic examination
Adhesion
Avidin
Throughput
Biophysics
Optical tweezers
Polystyrenes
Bond strength (materials)
Centrifuges
Colloids
Optical waveguides
Biotin
Biosensors
Washing
Particles (particulate matter)
Assays
Atomic force microscopy
Polymers

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Surfaces, Coatings and Films
  • Colloid and Surface Chemistry

Cite this

Adhesion force measurements on functionalized microbeads : An in-depth comparison of computer controlled micropipette and fluidic force microscopy. / Gerecsei, Tamás; Erdődi, István; Peter, Beatrix; Hős, Csaba; Kurunczi, Sándor; Derényi, I.; Szabó, B.; Horváth, R.

In: Journal of Colloid And Interface Science, Vol. 555, 01.11.2019, p. 245-253.

Research output: Contribution to journalArticle

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