Adenylyl cyclase plays a regulatory role in development, stress resistance and secondary metabolism in Fusarium fujikuroi

Jorge García-Martínez, A. Ádám, Javier Avalos

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The ascomycete fungus Fusarium fujikuroi (Gibberella fujikuroi MP-C) produces secondary metabolites of biotechnological interest, such as gibberellins, bikaverin, and carotenoids. Production of these metabolites is regulated by nitrogen availability and, in a specific manner, by other environmental signals, such as light in the case of the carotenoid pathway. A complex regulatory network controlling these processes is recently emerging from the alterations of metabolite production found through the mutation of different regulatory genes. Here we show the effect of the targeted mutation of the acyA gene of F. fujikuroi, coding for adenylyl cyclase. Mutants lacking the catalytic domain of the AcyA protein showed different phenotypic alterations, including reduced growth, enhanced production of unidentified red pigments, reduced production of gibberellins and partially derepressed carotenoid biosynthesis in the dark. The phenotype differs in some aspects from that of similar mutants of the close relatives F. proliferatum and F. verticillioides: contrary to what was observed in these species, ΔacyA mutants of F. fujikuroi showed enhanced sensitivity to oxidative stress (H 2O 2), but no change in heavy metal resistance or in the ability to colonize tomato tissue, indicating a high versatility in the regulatory roles played by cAMP in this fungal group.

Original languageEnglish
Article numbere28849
JournalPLoS One
Volume7
Issue number1
DOIs
Publication statusPublished - Jan 26 2012

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Secondary Metabolism
adenylate cyclase
Fusarium fujikuroi
Fusarium
Carotenoids
Adenylyl Cyclases
stress tolerance
Gibberellins
carotenoids
Metabolites
gibberellins
mutants
metabolism
Gibberella
metabolites
mutation
Ascomycota
Mutation
metal tolerance
Genes

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Adenylyl cyclase plays a regulatory role in development, stress resistance and secondary metabolism in Fusarium fujikuroi. / García-Martínez, Jorge; Ádám, A.; Avalos, Javier.

In: PLoS One, Vol. 7, No. 1, e28849, 26.01.2012.

Research output: Contribution to journalArticle

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