Adenovirus infection dramatically augments lipopolysaccharide-induced TNF production and sensitizes to lethal shock

György Fejér, Katalin Szalay, Ildikó Gyory, Mária Fejes, E. Kúsz, Saviana Nedieanu, T. Páli, Tibor Schmidt, Botond Siklódi, G. Lazar, György Lázár, E. Duda

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

We observed a remarkable synergism of adenoviruses and LPS in triggering the production of TNF in intact animals. We found that in mice pre-exposed to adenoviruses, LPS injections generated extremely high levels of TNF with altered kinetics. The elevated TNF synthesis stemmed mostly from posttranscriptional up-regulation of TNF production, although transcription of the TNF gene was also induced. Adenoviruses and LPS exhibited a significant but less dramatic synergism in the induction of IL-6, IFN-γ, and NO. Only marginal changes were detected in the synthesis of a panel of other cytokines. Different serotypes of the virus showed practically identical effects. As deletion mutants lacking indispensable viral genes or UV inactivated virions exhibited similar activities as the infectious, wild-type virus, it seems unlikely that the viral genome plays any significant role in the phenomenon. Published data indicate that other viruses also show some kind of synergism with LPS, although by different cellular mechanisms. T cells and their IFN-γ production-crucial in the synergism of influenza viruses and LPS-were dispensable in our experiments. We suggest that the phenomenon is probably a general one: an overlap between different molecular mechanisms detecting bacterial and viral pathogens and inducing mediators of nonspecific cell-mediated host defense. The synergism of viruses and LPS (bacteria) could be a concern in medical practice as well as in gene therapy experiments with high doses of recombinant adenoviruses.

Original languageEnglish
Pages (from-to)1498-1506
Number of pages9
JournalJournal of Immunology
Volume175
Issue number3
Publication statusPublished - Aug 1 2005

Fingerprint

Adenoviridae Infections
Adenoviridae
Lipopolysaccharides
Shock
Viruses
Viral Genes
Viral Genome
Orthomyxoviridae
Genetic Therapy
Virion
Interleukin-6
Up-Regulation
Cytokines
Bacteria
T-Lymphocytes
Injections
Genes

ASJC Scopus subject areas

  • Immunology

Cite this

Adenovirus infection dramatically augments lipopolysaccharide-induced TNF production and sensitizes to lethal shock. / Fejér, György; Szalay, Katalin; Gyory, Ildikó; Fejes, Mária; Kúsz, E.; Nedieanu, Saviana; Páli, T.; Schmidt, Tibor; Siklódi, Botond; Lazar, G.; Lázár, György; Duda, E.

In: Journal of Immunology, Vol. 175, No. 3, 01.08.2005, p. 1498-1506.

Research output: Contribution to journalArticle

Fejér, G, Szalay, K, Gyory, I, Fejes, M, Kúsz, E, Nedieanu, S, Páli, T, Schmidt, T, Siklódi, B, Lazar, G, Lázár, G & Duda, E 2005, 'Adenovirus infection dramatically augments lipopolysaccharide-induced TNF production and sensitizes to lethal shock', Journal of Immunology, vol. 175, no. 3, pp. 1498-1506.
Fejér, György ; Szalay, Katalin ; Gyory, Ildikó ; Fejes, Mária ; Kúsz, E. ; Nedieanu, Saviana ; Páli, T. ; Schmidt, Tibor ; Siklódi, Botond ; Lazar, G. ; Lázár, György ; Duda, E. / Adenovirus infection dramatically augments lipopolysaccharide-induced TNF production and sensitizes to lethal shock. In: Journal of Immunology. 2005 ; Vol. 175, No. 3. pp. 1498-1506.
@article{6ed3e1a4d76d4173a461c5f5d3728586,
title = "Adenovirus infection dramatically augments lipopolysaccharide-induced TNF production and sensitizes to lethal shock",
abstract = "We observed a remarkable synergism of adenoviruses and LPS in triggering the production of TNF in intact animals. We found that in mice pre-exposed to adenoviruses, LPS injections generated extremely high levels of TNF with altered kinetics. The elevated TNF synthesis stemmed mostly from posttranscriptional up-regulation of TNF production, although transcription of the TNF gene was also induced. Adenoviruses and LPS exhibited a significant but less dramatic synergism in the induction of IL-6, IFN-γ, and NO. Only marginal changes were detected in the synthesis of a panel of other cytokines. Different serotypes of the virus showed practically identical effects. As deletion mutants lacking indispensable viral genes or UV inactivated virions exhibited similar activities as the infectious, wild-type virus, it seems unlikely that the viral genome plays any significant role in the phenomenon. Published data indicate that other viruses also show some kind of synergism with LPS, although by different cellular mechanisms. T cells and their IFN-γ production-crucial in the synergism of influenza viruses and LPS-were dispensable in our experiments. We suggest that the phenomenon is probably a general one: an overlap between different molecular mechanisms detecting bacterial and viral pathogens and inducing mediators of nonspecific cell-mediated host defense. The synergism of viruses and LPS (bacteria) could be a concern in medical practice as well as in gene therapy experiments with high doses of recombinant adenoviruses.",
author = "Gy{\"o}rgy Fej{\'e}r and Katalin Szalay and Ildik{\'o} Gyory and M{\'a}ria Fejes and E. K{\'u}sz and Saviana Nedieanu and T. P{\'a}li and Tibor Schmidt and Botond Sikl{\'o}di and G. Lazar and Gy{\"o}rgy L{\'a}z{\'a}r and E. Duda",
year = "2005",
month = "8",
day = "1",
language = "English",
volume = "175",
pages = "1498--1506",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "3",

}

TY - JOUR

T1 - Adenovirus infection dramatically augments lipopolysaccharide-induced TNF production and sensitizes to lethal shock

AU - Fejér, György

AU - Szalay, Katalin

AU - Gyory, Ildikó

AU - Fejes, Mária

AU - Kúsz, E.

AU - Nedieanu, Saviana

AU - Páli, T.

AU - Schmidt, Tibor

AU - Siklódi, Botond

AU - Lazar, G.

AU - Lázár, György

AU - Duda, E.

PY - 2005/8/1

Y1 - 2005/8/1

N2 - We observed a remarkable synergism of adenoviruses and LPS in triggering the production of TNF in intact animals. We found that in mice pre-exposed to adenoviruses, LPS injections generated extremely high levels of TNF with altered kinetics. The elevated TNF synthesis stemmed mostly from posttranscriptional up-regulation of TNF production, although transcription of the TNF gene was also induced. Adenoviruses and LPS exhibited a significant but less dramatic synergism in the induction of IL-6, IFN-γ, and NO. Only marginal changes were detected in the synthesis of a panel of other cytokines. Different serotypes of the virus showed practically identical effects. As deletion mutants lacking indispensable viral genes or UV inactivated virions exhibited similar activities as the infectious, wild-type virus, it seems unlikely that the viral genome plays any significant role in the phenomenon. Published data indicate that other viruses also show some kind of synergism with LPS, although by different cellular mechanisms. T cells and their IFN-γ production-crucial in the synergism of influenza viruses and LPS-were dispensable in our experiments. We suggest that the phenomenon is probably a general one: an overlap between different molecular mechanisms detecting bacterial and viral pathogens and inducing mediators of nonspecific cell-mediated host defense. The synergism of viruses and LPS (bacteria) could be a concern in medical practice as well as in gene therapy experiments with high doses of recombinant adenoviruses.

AB - We observed a remarkable synergism of adenoviruses and LPS in triggering the production of TNF in intact animals. We found that in mice pre-exposed to adenoviruses, LPS injections generated extremely high levels of TNF with altered kinetics. The elevated TNF synthesis stemmed mostly from posttranscriptional up-regulation of TNF production, although transcription of the TNF gene was also induced. Adenoviruses and LPS exhibited a significant but less dramatic synergism in the induction of IL-6, IFN-γ, and NO. Only marginal changes were detected in the synthesis of a panel of other cytokines. Different serotypes of the virus showed practically identical effects. As deletion mutants lacking indispensable viral genes or UV inactivated virions exhibited similar activities as the infectious, wild-type virus, it seems unlikely that the viral genome plays any significant role in the phenomenon. Published data indicate that other viruses also show some kind of synergism with LPS, although by different cellular mechanisms. T cells and their IFN-γ production-crucial in the synergism of influenza viruses and LPS-were dispensable in our experiments. We suggest that the phenomenon is probably a general one: an overlap between different molecular mechanisms detecting bacterial and viral pathogens and inducing mediators of nonspecific cell-mediated host defense. The synergism of viruses and LPS (bacteria) could be a concern in medical practice as well as in gene therapy experiments with high doses of recombinant adenoviruses.

UR - http://www.scopus.com/inward/record.url?scp=22544441582&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=22544441582&partnerID=8YFLogxK

M3 - Article

VL - 175

SP - 1498

EP - 1506

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 3

ER -