Adenosine receptors mediate both contractile and relaxant effects of adenosine in main pulmonary artery of guinea pigs

A. József Szentmiklósi, Anikó Ujfalusi, Ágnes Cseppento, Klára Nosztray, Peter Kovacs, J. Szabó

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

In guinea pig main pulmonary artery precontracted with noradrenaline, adenosine exerted an initial phasic contraction followed by a tonic contraction and a slow relaxation. After selective blockade by 1,3-dipropyl-8-cyclopentylxanthine (DPCPX: 10 nM) of A1 receptors, adenosine only elicited a rapid relaxation. This initial response was characterized by use of adenosine (AR) and its analogues N6-cyclopentyl-adenosine (CPA), R-N6-phenyllsopropyladeno-sine (R-PIA), 2-chloroadenosine (CADO), 5′-N-ethyl-carboxamidoadenosine(NECA), N6-2-(4-aminophenyl) ethyl adenosine (APNEA) and 2-p-((carboxyethyl)phenethylamino)-5′-carboxamidoadenosine (CGS 21 680). The order of potency of the adenosine analogues for purine-induced phasic contraction was CPA > R-PIA > NECA = APNEA > AR > CGS 21 680 suggesting the involvement of activation of A1 type adenosine receptors in the contraction phase. DPCPX antagonized the CPA-induced contraction with a pA2 = 9.27 ± 0.26, but the Schild plot slope parameter was significantly lower than unity (0.58 ± 0.09). In contrast, in electrically driven guinea pig atrial myocardium (a tissue reported to possess A1 receptors), the DPCPX-CPA antagonism was purely competitive (pA2 = 8.95 ± 0.06; slope = 0.93 ± 0.06). In the presence of 300 nM DPCPX, the rank order of potency for the purine-induced fast relaxation was NECA > CADO = AR > CGS 21 680 = R-PIA > CPA. The NECA- and adenosine-induced relaxation was influenced neither by 300 nM CP 66 713 (an antagonist at A2a receptors), nor by endothelial removal and inhibition of nitric oxide synthase (100 μM NG-nitro-L-arginine: L-NOARG). The adenosine-induced relaxation was antagonized by 8-phenyltheophylline (8-PT), a potent A1/A2 antagonist. However, the rapid relaxation elicited by adenosine in the presence of 8-PT, was reversed and contraction developed. It is concluded that adenosine causes contraction via dual action on A1 adenosine receptors and on xanthine-resistant sites. Our experiments with APNEA (a prototypic A3 receptor agonist) did not support the suggestion that A3 receptors are implicated in the xanthine-resistant component of adenosine-induced contraction, as DPCPX (300 nM) completely abolished and even reversed the APNEA-induced contraction. In addition, cromolyn (a mast cell stabilizing agent) did not influence the xanthine-resistant contraction induced by adenosine in the presence of DPCPX, 8-PT and dipyridamole (an adenosine uptake inhibitor). On the basis of the rank order of agonist potency, the receptors involved in the adenosine-induced rapid relaxation most likely is of the A2b subtype. The opposing action of the xanthine-resistant contraction, however, did not allow a definitive pharmacological characterization of the receptor mediating relaxation.

Original languageEnglish
Pages (from-to)417-425
Number of pages9
JournalNaunyn-Schmiedeberg's Archives of Pharmacology
Volume351
Issue number4
DOIs
Publication statusPublished - Apr 1995

Fingerprint

Purinergic P1 Receptors
Adenosine
Pulmonary Artery
Guinea Pigs
Xanthine
Adenosine A1 Receptors
Nitroarginine
2-Chloroadenosine
Cromolyn Sodium

Keywords

  • Adenosine A receptors
  • Adenosine A receptors
  • Adenosine A receptors
  • Endothelium
  • Pulmonary artery
  • Smooth muscle regulation (vascular)
  • Xanthine-resistant contraction

ASJC Scopus subject areas

  • Pharmacology

Cite this

Adenosine receptors mediate both contractile and relaxant effects of adenosine in main pulmonary artery of guinea pigs. / Szentmiklósi, A. József; Ujfalusi, Anikó; Cseppento, Ágnes; Nosztray, Klára; Kovacs, Peter; Szabó, J.

In: Naunyn-Schmiedeberg's Archives of Pharmacology, Vol. 351, No. 4, 04.1995, p. 417-425.

Research output: Contribution to journalArticle

Szentmiklósi, A. József ; Ujfalusi, Anikó ; Cseppento, Ágnes ; Nosztray, Klára ; Kovacs, Peter ; Szabó, J. / Adenosine receptors mediate both contractile and relaxant effects of adenosine in main pulmonary artery of guinea pigs. In: Naunyn-Schmiedeberg's Archives of Pharmacology. 1995 ; Vol. 351, No. 4. pp. 417-425.
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T1 - Adenosine receptors mediate both contractile and relaxant effects of adenosine in main pulmonary artery of guinea pigs

AU - Szentmiklósi, A. József

AU - Ujfalusi, Anikó

AU - Cseppento, Ágnes

AU - Nosztray, Klára

AU - Kovacs, Peter

AU - Szabó, J.

PY - 1995/4

Y1 - 1995/4

N2 - In guinea pig main pulmonary artery precontracted with noradrenaline, adenosine exerted an initial phasic contraction followed by a tonic contraction and a slow relaxation. After selective blockade by 1,3-dipropyl-8-cyclopentylxanthine (DPCPX: 10 nM) of A1 receptors, adenosine only elicited a rapid relaxation. This initial response was characterized by use of adenosine (AR) and its analogues N6-cyclopentyl-adenosine (CPA), R-N6-phenyllsopropyladeno-sine (R-PIA), 2-chloroadenosine (CADO), 5′-N-ethyl-carboxamidoadenosine(NECA), N6-2-(4-aminophenyl) ethyl adenosine (APNEA) and 2-p-((carboxyethyl)phenethylamino)-5′-carboxamidoadenosine (CGS 21 680). The order of potency of the adenosine analogues for purine-induced phasic contraction was CPA > R-PIA > NECA = APNEA > AR > CGS 21 680 suggesting the involvement of activation of A1 type adenosine receptors in the contraction phase. DPCPX antagonized the CPA-induced contraction with a pA2 = 9.27 ± 0.26, but the Schild plot slope parameter was significantly lower than unity (0.58 ± 0.09). In contrast, in electrically driven guinea pig atrial myocardium (a tissue reported to possess A1 receptors), the DPCPX-CPA antagonism was purely competitive (pA2 = 8.95 ± 0.06; slope = 0.93 ± 0.06). In the presence of 300 nM DPCPX, the rank order of potency for the purine-induced fast relaxation was NECA > CADO = AR > CGS 21 680 = R-PIA > CPA. The NECA- and adenosine-induced relaxation was influenced neither by 300 nM CP 66 713 (an antagonist at A2a receptors), nor by endothelial removal and inhibition of nitric oxide synthase (100 μM NG-nitro-L-arginine: L-NOARG). The adenosine-induced relaxation was antagonized by 8-phenyltheophylline (8-PT), a potent A1/A2 antagonist. However, the rapid relaxation elicited by adenosine in the presence of 8-PT, was reversed and contraction developed. It is concluded that adenosine causes contraction via dual action on A1 adenosine receptors and on xanthine-resistant sites. Our experiments with APNEA (a prototypic A3 receptor agonist) did not support the suggestion that A3 receptors are implicated in the xanthine-resistant component of adenosine-induced contraction, as DPCPX (300 nM) completely abolished and even reversed the APNEA-induced contraction. In addition, cromolyn (a mast cell stabilizing agent) did not influence the xanthine-resistant contraction induced by adenosine in the presence of DPCPX, 8-PT and dipyridamole (an adenosine uptake inhibitor). On the basis of the rank order of agonist potency, the receptors involved in the adenosine-induced rapid relaxation most likely is of the A2b subtype. The opposing action of the xanthine-resistant contraction, however, did not allow a definitive pharmacological characterization of the receptor mediating relaxation.

AB - In guinea pig main pulmonary artery precontracted with noradrenaline, adenosine exerted an initial phasic contraction followed by a tonic contraction and a slow relaxation. After selective blockade by 1,3-dipropyl-8-cyclopentylxanthine (DPCPX: 10 nM) of A1 receptors, adenosine only elicited a rapid relaxation. This initial response was characterized by use of adenosine (AR) and its analogues N6-cyclopentyl-adenosine (CPA), R-N6-phenyllsopropyladeno-sine (R-PIA), 2-chloroadenosine (CADO), 5′-N-ethyl-carboxamidoadenosine(NECA), N6-2-(4-aminophenyl) ethyl adenosine (APNEA) and 2-p-((carboxyethyl)phenethylamino)-5′-carboxamidoadenosine (CGS 21 680). The order of potency of the adenosine analogues for purine-induced phasic contraction was CPA > R-PIA > NECA = APNEA > AR > CGS 21 680 suggesting the involvement of activation of A1 type adenosine receptors in the contraction phase. DPCPX antagonized the CPA-induced contraction with a pA2 = 9.27 ± 0.26, but the Schild plot slope parameter was significantly lower than unity (0.58 ± 0.09). In contrast, in electrically driven guinea pig atrial myocardium (a tissue reported to possess A1 receptors), the DPCPX-CPA antagonism was purely competitive (pA2 = 8.95 ± 0.06; slope = 0.93 ± 0.06). In the presence of 300 nM DPCPX, the rank order of potency for the purine-induced fast relaxation was NECA > CADO = AR > CGS 21 680 = R-PIA > CPA. The NECA- and adenosine-induced relaxation was influenced neither by 300 nM CP 66 713 (an antagonist at A2a receptors), nor by endothelial removal and inhibition of nitric oxide synthase (100 μM NG-nitro-L-arginine: L-NOARG). The adenosine-induced relaxation was antagonized by 8-phenyltheophylline (8-PT), a potent A1/A2 antagonist. However, the rapid relaxation elicited by adenosine in the presence of 8-PT, was reversed and contraction developed. It is concluded that adenosine causes contraction via dual action on A1 adenosine receptors and on xanthine-resistant sites. Our experiments with APNEA (a prototypic A3 receptor agonist) did not support the suggestion that A3 receptors are implicated in the xanthine-resistant component of adenosine-induced contraction, as DPCPX (300 nM) completely abolished and even reversed the APNEA-induced contraction. In addition, cromolyn (a mast cell stabilizing agent) did not influence the xanthine-resistant contraction induced by adenosine in the presence of DPCPX, 8-PT and dipyridamole (an adenosine uptake inhibitor). On the basis of the rank order of agonist potency, the receptors involved in the adenosine-induced rapid relaxation most likely is of the A2b subtype. The opposing action of the xanthine-resistant contraction, however, did not allow a definitive pharmacological characterization of the receptor mediating relaxation.

KW - Adenosine A receptors

KW - Adenosine A receptors

KW - Adenosine A receptors

KW - Endothelium

KW - Pulmonary artery

KW - Smooth muscle regulation (vascular)

KW - Xanthine-resistant contraction

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