Adenosine A2A receptor activation reduces lung injury in trauma/hemorrhagic shock

György Haskó, Da Zhong Xu, Qi Lu, Zoltán H. Németh, Jondavid Jabush, Tamara L. Berezina, Sergey B. Zaets, Balázs Csóka, Edwin A. Deitch

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Objective: Hemorrhagic shock and resuscitation trigger a global ischemia/reperfusion phenomenon, in which various inflammatory processes critically contribute to the ensuing tissue damage. Adenosine is an endogenous nucleoside that is released during shock. Activation of adenosine A2A receptors can broadly inactivate inflammatory cascades. The current study was designed to evaluate the effect of A2A receptor activation on organ injury and inflammation in the setting of global ischemia/reperfusion elicited by trauma/hemorrhagic shock and resuscitation. Design: Prospective animal study with concurrent control. Setting: Small animal laboratory. Subjects: Adult male Sprague-Dawley rats. Interventions: The rats were subjected to a laparotomy (trauma) and 90 mins of hemorrhagic shock or trauma/sham shock. The selective A2A receptor agonist CGS-21680 (2-p-(2-carboxyethyl) phenethylamino-5′-N-ethyl-carboxamidoadenosine; 0.5 mg/kg) or its vehicle was injected 30 mins before shock or immediately after resuscitation. At 3 hrs following resuscitation, animals were killed and tissue was harvested for analysis. Lung permeability and pulmonary myeloperoxidase levels were used to quantitate lung injury. Intestinal injury was determined by histologic analysis of terminal ileum. Red blood cell deformability was measured by a laser-assisted ektacytometer. In this assay, a decrease in the elongation index is a marker of decreased red blood cell deformability. Measurements and Main Results: Pretreatment with CGS-21680 protected the lung but not the gut against shock-induced injury and prevented the shock-induced decrease in red blood cell deformability. Posttreatment with CGS-21680 ameliorated shock-induced lung injury but failed to prevent gut injury and preserve red blood cell deformability. Conclusion: A2A receptor agonists may represent a novel therapeutic approach in preventing organ injury following trauma/ hemorrhagic shock.

Original languageEnglish
Pages (from-to)1119-1125
Number of pages7
JournalCritical care medicine
Volume34
Issue number4
DOIs
Publication statusPublished - Apr 1 2006

Keywords

  • Hypoxia
  • Inflammation
  • Ischemia
  • Neutrophils
  • Reperfusion

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine

Fingerprint Dive into the research topics of 'Adenosine A<sub>2A</sub> receptor activation reduces lung injury in trauma/hemorrhagic shock'. Together they form a unique fingerprint.

  • Cite this

    Haskó, G., Xu, D. Z., Lu, Q., Németh, Z. H., Jabush, J., Berezina, T. L., Zaets, S. B., Csóka, B., & Deitch, E. A. (2006). Adenosine A2A receptor activation reduces lung injury in trauma/hemorrhagic shock. Critical care medicine, 34(4), 1119-1125. https://doi.org/10.1097/01.CCM.0000206467.19509.C6