Addressing the optimal silver content in bioactive glass systems in terms of BSA adsorption

Klára Magyari, Cristina Gruian, Béla Varga, Raluca Ciceo-Lucacel, Teodora Radu, Heinz Jürgen Steinhoff, G. Váró, Viorica Simon, Lucian Baia

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Bioactive glasses doped with silver are aimed to minimize the risk of microbial contamination; therefore, the influence of silver on the bioactive properties is intensely investigated. However, information related to the role played by silver, when added to the bioactive glass composition, on biocompatibility properties is scarce. This aspect is essential as long as the silver content can influence blood protein adsorption onto the surface of the glass, thus affecting the material's biocompatibility. Therefore, from the perspective of the biocompatibility standpoint, the finding of an optimal silver content in a bioactive glass is an extremely important issue. In this study, silver-doped bioactive glasses were prepared by a melt-derived technique, which eliminates the pores' influence in the protein adsorption process. The obtained glasses were characterized by X-ray diffraction, UV-vis, X-ray photoelectron (XPS) and Fourier transform infrared (FT-IR) spectroscopy; afterwards, they were investigated in terms of protein adsorption. Both UV-vis and XPS spectroscopy revealed the presence of Ag+ ions in all silver containing samples. By increasing the silver content, metallic Ag0 appears, the highest amount being observed for the sample with 1 mol% AgO2. Electron paramagnetic resonance measurements evidenced that the amount of spin-labeled serum albumin attached to the surface increases with the silver content. The results obtained by analyzing the information derived from atomic force microscopy and FT-IR measurements indicate that the occurrence of metallic Ag0 in the samples' structure influences the secondary structure of the adsorbed protein. Based on the results derived from the protein response upon interaction with the investigated glass calcium-phosphate based system, the optimal silver oxide concentration was determined for which the secondary structure of the adsorbed protein is similar with that of the free one. This concentration was found to be 0.5 mol%. This journal is

Original languageEnglish
Pages (from-to)5799-5808
Number of pages10
JournalJournal of Materials Chemistry B
Volume2
Issue number35
DOIs
Publication statusPublished - Sep 21 2014

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Bioactive glass
Silver
Adsorption
Glass
Proteins
Biocompatibility
Secondary Protein Structure
X ray photoelectron spectroscopy
Silver oxides
Atomic Force Microscopy
Electron Spin Resonance Spectroscopy
Calcium phosphate
Fourier Analysis
Fourier Transform Infrared Spectroscopy
Photoelectrons
Serum Albumin
X-Ray Diffraction
Fourier transform infrared spectroscopy
Paramagnetic resonance
Blood Proteins

ASJC Scopus subject areas

  • Chemistry(all)
  • Biomedical Engineering
  • Medicine(all)
  • Materials Science(all)

Cite this

Magyari, K., Gruian, C., Varga, B., Ciceo-Lucacel, R., Radu, T., Steinhoff, H. J., ... Baia, L. (2014). Addressing the optimal silver content in bioactive glass systems in terms of BSA adsorption. Journal of Materials Chemistry B, 2(35), 5799-5808. https://doi.org/10.1039/c4tb00733f

Addressing the optimal silver content in bioactive glass systems in terms of BSA adsorption. / Magyari, Klára; Gruian, Cristina; Varga, Béla; Ciceo-Lucacel, Raluca; Radu, Teodora; Steinhoff, Heinz Jürgen; Váró, G.; Simon, Viorica; Baia, Lucian.

In: Journal of Materials Chemistry B, Vol. 2, No. 35, 21.09.2014, p. 5799-5808.

Research output: Contribution to journalArticle

Magyari, K, Gruian, C, Varga, B, Ciceo-Lucacel, R, Radu, T, Steinhoff, HJ, Váró, G, Simon, V & Baia, L 2014, 'Addressing the optimal silver content in bioactive glass systems in terms of BSA adsorption', Journal of Materials Chemistry B, vol. 2, no. 35, pp. 5799-5808. https://doi.org/10.1039/c4tb00733f
Magyari K, Gruian C, Varga B, Ciceo-Lucacel R, Radu T, Steinhoff HJ et al. Addressing the optimal silver content in bioactive glass systems in terms of BSA adsorption. Journal of Materials Chemistry B. 2014 Sep 21;2(35):5799-5808. https://doi.org/10.1039/c4tb00733f
Magyari, Klára ; Gruian, Cristina ; Varga, Béla ; Ciceo-Lucacel, Raluca ; Radu, Teodora ; Steinhoff, Heinz Jürgen ; Váró, G. ; Simon, Viorica ; Baia, Lucian. / Addressing the optimal silver content in bioactive glass systems in terms of BSA adsorption. In: Journal of Materials Chemistry B. 2014 ; Vol. 2, No. 35. pp. 5799-5808.
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