Additives enhancing the catalytic properties of lipase from burkholderia cepacia immobilized on mixed-function-grafted mesoporous silica gel

Emese Abaházi, Zoltán Boros, L. Poppe

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Effects of various additives on the lipase from Burkholderia cepacia (BcL) immobilized on mixed-function-grafted mesoporous silica gel support by hydrophobic adsorption and covalent attachment were investigated. Catalytic properties of the immobilized biocatalysts were characterized in kinetic resolution of racemic 1-phenylethanol (rac-1a) and 1-(thiophen-2-yl)ethan-1-ol (rac-1b). Screening of more than 40 additives showed significantly enhanced productivity of immobilized BcL with several additives such as PEGs, oleic acid and polyvinyl alcohol. Effects of substrate concentration and temperature between 0-100 °C on kinetic resolution of rac-1a were studied with the best adsorbed BcLs containing PEG 20 k or PVA 18-88 additives in continuous-flow packed-bed reactor. The optimum temperature of lipase activity for BcL co-immobilized with PEG 20k found at around 30 °C determined in the continuous-flow system increased remarkably to around 80 °C for BcL co-immobilized with PVA 18-88.

Original languageEnglish
Pages (from-to)9818-9837
Number of pages20
JournalMolecules
Volume19
Issue number7
DOIs
Publication statusPublished - 2014

Fingerprint

Burkholderia cepacia
Silica Gel
silica gel
Lipase
Polyethylene glycols
Polyvinyl Alcohol
Temperature
Oleic Acid
Adsorption
Kinetics
oleic acid
polyvinyl alcohol
kinetics
Packed beds
Enzymes
productivity
attachment
beds
Screening
alcohols

Keywords

  • Activity enhancement
  • Additive
  • Adsorption
  • Burkholderia cepacia lipase
  • Continuous-flow packed-bed reactor
  • Enzyme immobilization
  • Silica gel

ASJC Scopus subject areas

  • Organic Chemistry
  • Medicine(all)

Cite this

Additives enhancing the catalytic properties of lipase from burkholderia cepacia immobilized on mixed-function-grafted mesoporous silica gel. / Abaházi, Emese; Boros, Zoltán; Poppe, L.

In: Molecules, Vol. 19, No. 7, 2014, p. 9818-9837.

Research output: Contribution to journalArticle

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