Adaptation to tert-butyl hydroperoxide at a plasma membrane level in the fission yeast Schizosaccharomyces pombe parental strain and its t-BuOOH-resistant mutant

Nikoletta Kálmán, Zoltán Gazdag, Milan Čertík, J. Belágyi, Samy A. Selim, I. Pócsi, M. Pesti

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The one-gene mutant hyd1-190 of the fission yeast Schizosaccharomyces pombe displayed four-fold resistance to tert-butyl hydroperoxide (t-BuOOH) in comparison with its parental strain hyd+. The cells of hyd1-190 exhibited a quantitative alteration in the sterol content and hence in the fatty acid composition of the plasma membrane, reflected in a two-fold amphotericin B sensitivity, increased rigidity of the plasma membrane, revealed by an elevated (Δ7.9°C) phase-transition temperature, measured by means of electron paramagnetic resonance spectroscopy, and a significantly decreased uptake of glycerol. Treatment of the strains with a subinhibitory concentration (0.2mM) of t-BuOOH induced adaptation via modification of the sterol and fatty acid compositions, resulting in increased (Δ3.95°C) and decreased (Δ6.83°C) phase-transition temperatures of the hyd+ and hyd1-190 strains, respectively, in order to defend the cells against the consequences of t-BuOOH-induced external oxidative stress. However, in contrast with hyd+, hyd1-190 lacks the ability to adapt to t-BuOOH at a cell level.

Original languageEnglish
Pages (from-to)215-225
Number of pages11
JournalJournal of Basic Microbiology
Volume54
Issue number3
DOIs
Publication statusPublished - Mar 2014

Fingerprint

tert-Butylhydroperoxide
Schizosaccharomyces
Transition Temperature
Phase Transition
Cell Membrane
Sterols
Fatty Acids
Electron Spin Resonance Spectroscopy
Amphotericin B
Glycerol
Spectrum Analysis
Oxidative Stress
Genes

Keywords

  • Oxidative stress
  • Plasma membrane
  • Resistance
  • Tert-butyl hydroperoxide
  • Yeast

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology

Cite this

Adaptation to tert-butyl hydroperoxide at a plasma membrane level in the fission yeast Schizosaccharomyces pombe parental strain and its t-BuOOH-resistant mutant. / Kálmán, Nikoletta; Gazdag, Zoltán; Čertík, Milan; Belágyi, J.; Selim, Samy A.; Pócsi, I.; Pesti, M.

In: Journal of Basic Microbiology, Vol. 54, No. 3, 03.2014, p. 215-225.

Research output: Contribution to journalArticle

@article{0c68f7bed677415bbdb279faaf3563bd,
title = "Adaptation to tert-butyl hydroperoxide at a plasma membrane level in the fission yeast Schizosaccharomyces pombe parental strain and its t-BuOOH-resistant mutant",
abstract = "The one-gene mutant hyd1-190 of the fission yeast Schizosaccharomyces pombe displayed four-fold resistance to tert-butyl hydroperoxide (t-BuOOH) in comparison with its parental strain hyd+. The cells of hyd1-190 exhibited a quantitative alteration in the sterol content and hence in the fatty acid composition of the plasma membrane, reflected in a two-fold amphotericin B sensitivity, increased rigidity of the plasma membrane, revealed by an elevated (Δ7.9°C) phase-transition temperature, measured by means of electron paramagnetic resonance spectroscopy, and a significantly decreased uptake of glycerol. Treatment of the strains with a subinhibitory concentration (0.2mM) of t-BuOOH induced adaptation via modification of the sterol and fatty acid compositions, resulting in increased (Δ3.95°C) and decreased (Δ6.83°C) phase-transition temperatures of the hyd+ and hyd1-190 strains, respectively, in order to defend the cells against the consequences of t-BuOOH-induced external oxidative stress. However, in contrast with hyd+, hyd1-190 lacks the ability to adapt to t-BuOOH at a cell level.",
keywords = "Oxidative stress, Plasma membrane, Resistance, Tert-butyl hydroperoxide, Yeast",
author = "Nikoletta K{\'a}lm{\'a}n and Zolt{\'a}n Gazdag and Milan Čert{\'i}k and J. Bel{\'a}gyi and Selim, {Samy A.} and I. P{\'o}csi and M. Pesti",
year = "2014",
month = "3",
doi = "10.1002/jobm.201200580",
language = "English",
volume = "54",
pages = "215--225",
journal = "Journal of Basic Microbiology",
issn = "0233-111X",
publisher = "Wiley-VCH Verlag",
number = "3",

}

TY - JOUR

T1 - Adaptation to tert-butyl hydroperoxide at a plasma membrane level in the fission yeast Schizosaccharomyces pombe parental strain and its t-BuOOH-resistant mutant

AU - Kálmán, Nikoletta

AU - Gazdag, Zoltán

AU - Čertík, Milan

AU - Belágyi, J.

AU - Selim, Samy A.

AU - Pócsi, I.

AU - Pesti, M.

PY - 2014/3

Y1 - 2014/3

N2 - The one-gene mutant hyd1-190 of the fission yeast Schizosaccharomyces pombe displayed four-fold resistance to tert-butyl hydroperoxide (t-BuOOH) in comparison with its parental strain hyd+. The cells of hyd1-190 exhibited a quantitative alteration in the sterol content and hence in the fatty acid composition of the plasma membrane, reflected in a two-fold amphotericin B sensitivity, increased rigidity of the plasma membrane, revealed by an elevated (Δ7.9°C) phase-transition temperature, measured by means of electron paramagnetic resonance spectroscopy, and a significantly decreased uptake of glycerol. Treatment of the strains with a subinhibitory concentration (0.2mM) of t-BuOOH induced adaptation via modification of the sterol and fatty acid compositions, resulting in increased (Δ3.95°C) and decreased (Δ6.83°C) phase-transition temperatures of the hyd+ and hyd1-190 strains, respectively, in order to defend the cells against the consequences of t-BuOOH-induced external oxidative stress. However, in contrast with hyd+, hyd1-190 lacks the ability to adapt to t-BuOOH at a cell level.

AB - The one-gene mutant hyd1-190 of the fission yeast Schizosaccharomyces pombe displayed four-fold resistance to tert-butyl hydroperoxide (t-BuOOH) in comparison with its parental strain hyd+. The cells of hyd1-190 exhibited a quantitative alteration in the sterol content and hence in the fatty acid composition of the plasma membrane, reflected in a two-fold amphotericin B sensitivity, increased rigidity of the plasma membrane, revealed by an elevated (Δ7.9°C) phase-transition temperature, measured by means of electron paramagnetic resonance spectroscopy, and a significantly decreased uptake of glycerol. Treatment of the strains with a subinhibitory concentration (0.2mM) of t-BuOOH induced adaptation via modification of the sterol and fatty acid compositions, resulting in increased (Δ3.95°C) and decreased (Δ6.83°C) phase-transition temperatures of the hyd+ and hyd1-190 strains, respectively, in order to defend the cells against the consequences of t-BuOOH-induced external oxidative stress. However, in contrast with hyd+, hyd1-190 lacks the ability to adapt to t-BuOOH at a cell level.

KW - Oxidative stress

KW - Plasma membrane

KW - Resistance

KW - Tert-butyl hydroperoxide

KW - Yeast

UR - http://www.scopus.com/inward/record.url?scp=84894808807&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84894808807&partnerID=8YFLogxK

U2 - 10.1002/jobm.201200580

DO - 10.1002/jobm.201200580

M3 - Article

VL - 54

SP - 215

EP - 225

JO - Journal of Basic Microbiology

JF - Journal of Basic Microbiology

SN - 0233-111X

IS - 3

ER -