Adaptation of the ASTRIN staining technique for quantitative determination of the functional state of the cellular nucleus: Experiments on the nucleoli of eRO cells

Péter Kovács, György Csaba, László Kőhidai, Ottilie Török

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Abstract

Cytophotometric determination between 516 ≤ λ ≤ 745 nm of the nucleolar spectra of cells stained with the ASTRIN technique has enabled the quantitative assessment of stain binding affinity and its changes. The hormone-like active molecule γ-L-glutamyl-taurine, which acts at the nuclear level, gave rise to nucleolar activation, which could be precisely followed up by ASTRIN staining. It appears that the ASTRIN staining technique is suitable for the quantitative determination of changes in the functional state of the nucleus and nucleolus.

Original languageEnglish
Pages (from-to)95-98
Number of pages4
JournalActa histochemica
Volume81
Issue number1
DOIs
Publication statusPublished - Jan 1 1987

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ASJC Scopus subject areas

  • Histology
  • Cell Biology

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