Acute toxicity of selenate and selenite and their impacts on oxidative status, efflux pump activity, cellular and genetic parameters in earthworm Eisenia andrei

Sandra Ečimović, Mirna Velki, Rosemary Vuković, Ivna Štolfa Čamagajevac, Anja Petek, Rebeka Bošnjaković, Magdalena Grgić, P. Engelmann, Kornélia Bodó, Vlatka Filipović-Marijić, Dušica Ivanković, Marijana Erk, Tatjana Mijošek, Zdenko Lončarić

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Selenium (Se) is an essential element for humans, animals, and certain lower plants, but can be toxic at high concentration. Even though Se is potentially toxic, little information is available about the effects of Se on soil animals. The aim of this study was to assess the impact of different concentrations of two Se forms, selenate and selenite, on earthworm Eisenia andrei. In order to obtain comprehensive overview on the Se effects, different parameters were measured. Namely, acute toxicity, apoptosis, efflux pump activity, different enzymatic and non-enzymatic biomarkers (acetylcholinesterase, carboxylesterase, glutathione S-transferase, catalase, glutathione reductase and superoxide dismutase activities, lipid peroxidation level and GSH/GSSG ratio) and expression of genes involved in oxidative and immune response have been investigated. Additionally, measurement of metallothioneins concentration and concentration of Se in exposed earthworms has been also performed. The assessment of acute toxicity showed a greater sensitivity of E. andrei to selenite exposure, whereas Se concentration measurements in earthworms showed higher accumulation of selenate form. Both Se forms caused inhibition of the efflux pump activity. Decrease in superoxide dismutase activity and increase in lipid peroxidation and glutathione reductase activity indicate that Se has a significant impact on the oxidative status of earthworms. Selenate exposure caused an apoptotic-like cell death in the coelomocytes of exposed earthworms, whereas decreased mRNA levels of stress-related genes and antimicrobial factors were observed upon the exposure to selenite. The obtained data give insight into the effects of two most common forms of Se in soil on the earthworm E. andrei.

Original languageEnglish
Pages (from-to)307-318
Number of pages12
JournalChemosphere
Volume212
DOIs
Publication statusPublished - Dec 1 2018

Fingerprint

Selenic Acid
Selenious Acid
selenate
Oligochaeta
selenite
Selenium
selenium
earthworm
Toxicity
pump
Pumps
toxicity
Glutathione Reductase
Poisons
Cell death
Lipids
Lipid Peroxidation
Superoxide Dismutase
Animals
Soil

Keywords

  • Apoptosis
  • Biomarker responses
  • Efflux pump activity
  • Gene expression
  • Metallothioneins
  • Selenium toxicity

ASJC Scopus subject areas

  • Chemistry(all)
  • Environmental Chemistry

Cite this

Acute toxicity of selenate and selenite and their impacts on oxidative status, efflux pump activity, cellular and genetic parameters in earthworm Eisenia andrei. / Ečimović, Sandra; Velki, Mirna; Vuković, Rosemary; Štolfa Čamagajevac, Ivna; Petek, Anja; Bošnjaković, Rebeka; Grgić, Magdalena; Engelmann, P.; Bodó, Kornélia; Filipović-Marijić, Vlatka; Ivanković, Dušica; Erk, Marijana; Mijošek, Tatjana; Lončarić, Zdenko.

In: Chemosphere, Vol. 212, 01.12.2018, p. 307-318.

Research output: Contribution to journalArticle

Ečimović, S, Velki, M, Vuković, R, Štolfa Čamagajevac, I, Petek, A, Bošnjaković, R, Grgić, M, Engelmann, P, Bodó, K, Filipović-Marijić, V, Ivanković, D, Erk, M, Mijošek, T & Lončarić, Z 2018, 'Acute toxicity of selenate and selenite and their impacts on oxidative status, efflux pump activity, cellular and genetic parameters in earthworm Eisenia andrei', Chemosphere, vol. 212, pp. 307-318. https://doi.org/10.1016/j.chemosphere.2018.08.095
Ečimović, Sandra ; Velki, Mirna ; Vuković, Rosemary ; Štolfa Čamagajevac, Ivna ; Petek, Anja ; Bošnjaković, Rebeka ; Grgić, Magdalena ; Engelmann, P. ; Bodó, Kornélia ; Filipović-Marijić, Vlatka ; Ivanković, Dušica ; Erk, Marijana ; Mijošek, Tatjana ; Lončarić, Zdenko. / Acute toxicity of selenate and selenite and their impacts on oxidative status, efflux pump activity, cellular and genetic parameters in earthworm Eisenia andrei. In: Chemosphere. 2018 ; Vol. 212. pp. 307-318.
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AU - Petek, Anja

AU - Bošnjaković, Rebeka

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