Acute toxic effects of three pesticides on Pseudomonas putida monitored by microcalorimeter

Hui Lun Chen, Jun Yao, Fei Wang, Emilia Bramanti, Thomas Maskow, G. Záray

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A series of calorimetric experiments were performed to investigate the toxic effects of beta-cypermethrin (BCP), bensulfuron-methyl (BSM) and prometryne (PM) on Pseudomonas putida (P. putida). The metabolic action of P. putida on the three pesticides was studied by obtaining power-time curves. The growth of P. putida was inhibited completely in each case when the concentrations of pesticides were up to 80 μg mL- 1. The relationships between the inhibitory ratio (k) and doses of contaminants were approximately linear for the three pesticides. The total heat dissipated per milliliter (Qtotal) for the pesticides decreased during the course of the experiment. The OD600 of P. putida growth in the absence and presence of pesticides was also obtained. The power-time curves of P. putida growth coincided with its turbidity curves. This elucidates that microcalorimetric method agrees well with the routine microbiological method. Among these three pesticides, BSM was found to be the most toxic with an IC50 of 19.24 μg mL- 1 against P. putida. PM exhibited moderate virulence with an IC50 of 27.86 μg mL- 1 and BCP had the lowest toxicity with an IC50 of 39.64 μg mL- 1.

Original languageEnglish
Pages (from-to)157-163
Number of pages7
JournalJournal of Environmental Science and Health - Part B Pesticides, Food Contaminants, and Agricultural Wastes
Volume44
Issue number2
DOIs
Publication statusPublished - Feb 2009

Fingerprint

Pseudomonas putida
Poisons
Pesticides
pesticides
Prometryne
bensulfuron-methyl
prometryn
Inhibitory Concentration 50
inhibitory concentration 50
cypermethrin
Growth
Turbidity
turbidity
Toxicity
Virulence
Enthalpy
virulence
Hot Temperature
Experiments
Impurities

Keywords

  • Bensulfuron-methyl
  • Beta-cypermethrin
  • Microcalorimetry
  • Prometryne
  • Toxic effect

ASJC Scopus subject areas

  • Food Science
  • Pollution

Cite this

Acute toxic effects of three pesticides on Pseudomonas putida monitored by microcalorimeter. / Chen, Hui Lun; Yao, Jun; Wang, Fei; Bramanti, Emilia; Maskow, Thomas; Záray, G.

In: Journal of Environmental Science and Health - Part B Pesticides, Food Contaminants, and Agricultural Wastes, Vol. 44, No. 2, 02.2009, p. 157-163.

Research output: Contribution to journalArticle

@article{aef69efb887546dc85f12221108f109c,
title = "Acute toxic effects of three pesticides on Pseudomonas putida monitored by microcalorimeter",
abstract = "A series of calorimetric experiments were performed to investigate the toxic effects of beta-cypermethrin (BCP), bensulfuron-methyl (BSM) and prometryne (PM) on Pseudomonas putida (P. putida). The metabolic action of P. putida on the three pesticides was studied by obtaining power-time curves. The growth of P. putida was inhibited completely in each case when the concentrations of pesticides were up to 80 μg mL- 1. The relationships between the inhibitory ratio (k) and doses of contaminants were approximately linear for the three pesticides. The total heat dissipated per milliliter (Qtotal) for the pesticides decreased during the course of the experiment. The OD600 of P. putida growth in the absence and presence of pesticides was also obtained. The power-time curves of P. putida growth coincided with its turbidity curves. This elucidates that microcalorimetric method agrees well with the routine microbiological method. Among these three pesticides, BSM was found to be the most toxic with an IC50 of 19.24 μg mL- 1 against P. putida. PM exhibited moderate virulence with an IC50 of 27.86 μg mL- 1 and BCP had the lowest toxicity with an IC50 of 39.64 μg mL- 1.",
keywords = "Bensulfuron-methyl, Beta-cypermethrin, Microcalorimetry, Prometryne, Toxic effect",
author = "Chen, {Hui Lun} and Jun Yao and Fei Wang and Emilia Bramanti and Thomas Maskow and G. Z{\'a}ray",
year = "2009",
month = "2",
doi = "10.1080/03601230802599084",
language = "English",
volume = "44",
pages = "157--163",
journal = "Journal of Environmental Science and Health - Part B Pesticides, Food Contaminants, and Agricultural Wastes",
issn = "0360-1234",
publisher = "Taylor and Francis Ltd.",
number = "2",

}

TY - JOUR

T1 - Acute toxic effects of three pesticides on Pseudomonas putida monitored by microcalorimeter

AU - Chen, Hui Lun

AU - Yao, Jun

AU - Wang, Fei

AU - Bramanti, Emilia

AU - Maskow, Thomas

AU - Záray, G.

PY - 2009/2

Y1 - 2009/2

N2 - A series of calorimetric experiments were performed to investigate the toxic effects of beta-cypermethrin (BCP), bensulfuron-methyl (BSM) and prometryne (PM) on Pseudomonas putida (P. putida). The metabolic action of P. putida on the three pesticides was studied by obtaining power-time curves. The growth of P. putida was inhibited completely in each case when the concentrations of pesticides were up to 80 μg mL- 1. The relationships between the inhibitory ratio (k) and doses of contaminants were approximately linear for the three pesticides. The total heat dissipated per milliliter (Qtotal) for the pesticides decreased during the course of the experiment. The OD600 of P. putida growth in the absence and presence of pesticides was also obtained. The power-time curves of P. putida growth coincided with its turbidity curves. This elucidates that microcalorimetric method agrees well with the routine microbiological method. Among these three pesticides, BSM was found to be the most toxic with an IC50 of 19.24 μg mL- 1 against P. putida. PM exhibited moderate virulence with an IC50 of 27.86 μg mL- 1 and BCP had the lowest toxicity with an IC50 of 39.64 μg mL- 1.

AB - A series of calorimetric experiments were performed to investigate the toxic effects of beta-cypermethrin (BCP), bensulfuron-methyl (BSM) and prometryne (PM) on Pseudomonas putida (P. putida). The metabolic action of P. putida on the three pesticides was studied by obtaining power-time curves. The growth of P. putida was inhibited completely in each case when the concentrations of pesticides were up to 80 μg mL- 1. The relationships between the inhibitory ratio (k) and doses of contaminants were approximately linear for the three pesticides. The total heat dissipated per milliliter (Qtotal) for the pesticides decreased during the course of the experiment. The OD600 of P. putida growth in the absence and presence of pesticides was also obtained. The power-time curves of P. putida growth coincided with its turbidity curves. This elucidates that microcalorimetric method agrees well with the routine microbiological method. Among these three pesticides, BSM was found to be the most toxic with an IC50 of 19.24 μg mL- 1 against P. putida. PM exhibited moderate virulence with an IC50 of 27.86 μg mL- 1 and BCP had the lowest toxicity with an IC50 of 39.64 μg mL- 1.

KW - Bensulfuron-methyl

KW - Beta-cypermethrin

KW - Microcalorimetry

KW - Prometryne

KW - Toxic effect

UR - http://www.scopus.com/inward/record.url?scp=61649091350&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=61649091350&partnerID=8YFLogxK

U2 - 10.1080/03601230802599084

DO - 10.1080/03601230802599084

M3 - Article

C2 - 19130374

AN - SCOPUS:61649091350

VL - 44

SP - 157

EP - 163

JO - Journal of Environmental Science and Health - Part B Pesticides, Food Contaminants, and Agricultural Wastes

JF - Journal of Environmental Science and Health - Part B Pesticides, Food Contaminants, and Agricultural Wastes

SN - 0360-1234

IS - 2

ER -