Acute effects of cadmium on preovulatory serum FSH, LH, and prolactin levels and on ovulation and ovarian hormone secretion in estrous rats

Katalin Paksy, Bertalan Varga, Edit Horváth, Erzsébet Tátrai, György Ungváry

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

On the day of diestrus II CFY rats were given 5, 10, or 15 mg/kg cadmium chloride (CdCl2) or 1.0 mL/kg of 0.9% NaCl. On the next day a group of animals was anesthetized with pentobarbital and blood was collected from the aorta at 13:00, 15:00, 16:30, or 18:00 h. for FSH, LH, prolactin (PRL), progesterone (P), and estradiol-17β (E2) determination. On the day of the expected estrus, the second group of animals was anesthetized with pentobarbital and cannulas were inserted in one of the femoral arteries and veins, and in one of the utero-ovarian veins. Five-minute blood fractions were collected from the ovary for 40 min, and following the first blood samples, 10 IU hCG was injected iv. Ovarian venous outflow and blood pressure were continuously recorded. From the blood fractions, P and E2 were determined, and their secretion rates were calculated. In a third group of treated animals, the ovaries were excised for histological examination, and oviducts were flushed for counting oocytes. CdCl2 in the dose of 10 or 15 mg/kg increased the PRL serum levels at 13:00 h; it diminished FSH serum levels in the dose of 10 mg/kg and LH serum levels in the doses of 10 and 15 mg/kg at 15:00 h. The decrease in LH levels continued until 16:30 h in the dose of 10 mg/kg CdCl2. In estrous animals, CdCl2 did not influence the blood pressure and ovarian blood flow. In animals receiving 10 or 15 mg/kg CdCl2, a decrease in basal secretion of P occurred. The hCG induced a rapid rise in P secretion of controls; the 5 mg/kg CdCl2 diminished the effect of hCG, and the 10 and 15 mg/kg completely abolished it, but failed to influence the basal secretion of E2 and its response to hCG. CdCl2 prevented ovulation in 40% to 50% of the animals; however, when ovulation occurred, normal oocyte numbers were found. No alteration could be observed in the light microscopic histology of ovary.

Original languageEnglish
Pages (from-to)241-247
Number of pages7
JournalReproductive Toxicology
Volume3
Issue number4
DOIs
Publication statusPublished - 1989

Keywords

  • Cadmium chloride
  • FSH
  • LH
  • Ovary
  • Ovulation
  • Progesterone secretion
  • prolactin

ASJC Scopus subject areas

  • Toxicology

Fingerprint Dive into the research topics of 'Acute effects of cadmium on preovulatory serum FSH, LH, and prolactin levels and on ovulation and ovarian hormone secretion in estrous rats'. Together they form a unique fingerprint.

  • Cite this