Acute changes in urinary excretion of nitrite + nitrate do not necessarily predict renal vascular NO production

T. Suto, G. Losonczy, C. Qiu, C. Hill, L. Samsell, J. Ruby, N. Charon, R. Venuto, C. Baylis

Research output: Contribution to journalArticle

123 Citations (Scopus)

Abstract

NO2 + NO3 (NO(x)), the stable oxidation products of NO, and cGMP are widely accepted as indices of in vivo NO production. Whether acute changes in urinary excretion of nitrite + nitrate (U(NOX)V) can be taken to reflect acute changes in renal and/or systemic NO production is not known. The present studies were conducted in the conscious rat to investigate the effect on acute changes in U(NOx)V, of maneuvers that (a) enhance NO production and (b) act as diuretics. L-arginine (L-arg) and acetylcholine (Ach) produce equivalent NO dependent falls in renal vascular resistance (RVR), but a much greater increase in U(NOX)V is seen with L-arg. D-arg does not stimulate NO and has no renal vasodilatory effect, but produces a large rise in U(NOX)V, and SNP lowers BP but not RVR and results in a reduced U(NOX)V. None of the diuretics employed should stimulate the NO system or lower RVR; however, the proximally acting agents, acetazolamide and D-arg increased U(NOx)V, while the loop diuretic furosemide had little effect. H2O diuresis (a distal event) led to a fall in U(NOx)V. These data suggest that NO(x) is reabsorbed extensively in the proximal tubule and that inhibition of proximal reabsorption leads to an increase in U(NOx)V. Also, our results show that the relationship between U(NOx)V and U(cGMP)V is unpredictable. Therefore, we conclude that measurements of acute changes in U(NOx)V and/or U(cGMP)V should be interpreted cautiously, since they may reflect altered tubular handling of NO(x) rather than the acute activity of the systemic and/or renal NO systems.

Original languageEnglish
Pages (from-to)1272-1277
Number of pages6
JournalKidney International
Volume48
Issue number4
Publication statusPublished - 1995

Fingerprint

Nitrites
Nitrates
Blood Vessels
Kidney
Vascular Resistance
Diuretics
Sodium Potassium Chloride Symporter Inhibitors
Acetazolamide
Diuresis
Furosemide
Acetylcholine
Single Nucleotide Polymorphism
Arginine

ASJC Scopus subject areas

  • Nephrology

Cite this

Suto, T., Losonczy, G., Qiu, C., Hill, C., Samsell, L., Ruby, J., ... Baylis, C. (1995). Acute changes in urinary excretion of nitrite + nitrate do not necessarily predict renal vascular NO production. Kidney International, 48(4), 1272-1277.

Acute changes in urinary excretion of nitrite + nitrate do not necessarily predict renal vascular NO production. / Suto, T.; Losonczy, G.; Qiu, C.; Hill, C.; Samsell, L.; Ruby, J.; Charon, N.; Venuto, R.; Baylis, C.

In: Kidney International, Vol. 48, No. 4, 1995, p. 1272-1277.

Research output: Contribution to journalArticle

Suto, T, Losonczy, G, Qiu, C, Hill, C, Samsell, L, Ruby, J, Charon, N, Venuto, R & Baylis, C 1995, 'Acute changes in urinary excretion of nitrite + nitrate do not necessarily predict renal vascular NO production', Kidney International, vol. 48, no. 4, pp. 1272-1277.
Suto, T. ; Losonczy, G. ; Qiu, C. ; Hill, C. ; Samsell, L. ; Ruby, J. ; Charon, N. ; Venuto, R. ; Baylis, C. / Acute changes in urinary excretion of nitrite + nitrate do not necessarily predict renal vascular NO production. In: Kidney International. 1995 ; Vol. 48, No. 4. pp. 1272-1277.
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