Az akut és krónikus stressz hatása az Alzheimerkór patomechanizmusában szerepet játszó gének transzkripciójára

Translated title of the contribution: Acute and chronic stress induced changes in gene transcriptions related to Alzheimer's disease

Petra Sántha, M. Pákáski, Örsike Fazekas, Szabina Szucs, Eszter Klára Fodor, J. Kálmán, Sára Kálmán, Gyula Szabó, Z. Janka, János Kálmán

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Preclinical and clinical studies demonstrate that stress may be implicated in the risk of neurodegenerative diseases such as Alzheimer's disease (AD). Our study aimed to investigate the effects of acute and chronic immobilization stress (IS) on the gene transcriptions of β-actin, amyloid precursor protein (APP) and mitogen activated protein kinase-1 (MAPK-1), proteins related to synaptic plasticity and neuronal degeneration. Male Wistar rats were exposed to IS for five hours daily for 3 days (acute stress) or through 7-14-21 days (chronic stress). At the end of exposure periods, total RNA was purified from the cortex and hippocampus. The amounts of β-actin, APP and MAPK-1 mRNA were determined with real time PCR method. Our results indicate that the mRNA expression of β-actin and APP followed a U-shaped time-response curve. Both acute and chronic IS caused a significant increase in β-actin and MAPK-1 mRNA expression. Significant APP mRNA elevation was observed only by the 3rd week after RS. Our findings demonstrate that both acute and chronic IS lead to gene transcriptional changes of β-actin, APP and MAPK-1. These proteins maintain the normal function of the cytoskeleton and the synaptic plasticity. The above changes may lead to cognitive deterioration, and the development of AD.

Original languageHungarian
Pages (from-to)195-200
Number of pages6
JournalIdeggyógyászati szemle
Volume65
Issue number5-6
Publication statusPublished - May 30 2012

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Amyloid beta-Protein Precursor
Mitogen-Activated Protein Kinase 1
Actins
Alzheimer Disease
Immobilization
Messenger RNA
Neuronal Plasticity
Genes
Cytoskeleton
Neurodegenerative Diseases
Wistar Rats
Real-Time Polymerase Chain Reaction
Hippocampus
Proteins
RNA

ASJC Scopus subject areas

  • Clinical Neurology
  • Neurology

Cite this

Az akut és krónikus stressz hatása az Alzheimerkór patomechanizmusában szerepet játszó gének transzkripciójára. / Sántha, Petra; Pákáski, M.; Fazekas, Örsike; Szucs, Szabina; Fodor, Eszter Klára; Kálmán, J.; Kálmán, Sára; Szabó, Gyula; Janka, Z.; Kálmán, János.

In: Ideggyógyászati szemle, Vol. 65, No. 5-6, 30.05.2012, p. 195-200.

Research output: Contribution to journalArticle

Sántha, P, Pákáski, M, Fazekas, Ö, Szucs, S, Fodor, EK, Kálmán, J, Kálmán, S, Szabó, G, Janka, Z & Kálmán, J 2012, 'Az akut és krónikus stressz hatása az Alzheimerkór patomechanizmusában szerepet játszó gének transzkripciójára', Ideggyógyászati szemle, vol. 65, no. 5-6, pp. 195-200.
Sántha, Petra ; Pákáski, M. ; Fazekas, Örsike ; Szucs, Szabina ; Fodor, Eszter Klára ; Kálmán, J. ; Kálmán, Sára ; Szabó, Gyula ; Janka, Z. ; Kálmán, János. / Az akut és krónikus stressz hatása az Alzheimerkór patomechanizmusában szerepet játszó gének transzkripciójára. In: Ideggyógyászati szemle. 2012 ; Vol. 65, No. 5-6. pp. 195-200.
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