Activity-regulated RNA editing in select neuronal subfields in hippocampus

Ales Balik, Andrew C. Penn, Z. Nemoda, Ingo H. Greger

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

RNA editing by adensosine deaminases is a widespread mechanism to alter genetic information in metazoa. In addition to modifications in non-coding regions, editing contributes to diversification of protein function, in analogy to alternative splicing. However, although splicing programs respond to external signals, facilitating fine tuning and homeostasis of cellular functions, a similar regulation has not been described for RNA editing. Here, we show that the AMPA receptor R/G editing site is dynamically regulated in the hippocampus in response to activity. These changes are bi-directional, reversible and correlate with levels of the editase Adar2. This regulation is observed in the CA1 hippocampal subfield but not in CA3 and is thus subfield/celltype-specific. Moreover, alternative splicing of the flip/flop cassette downstream of the R/G site is closely linked to the editing state, which is regulated by Ca 2+. Our data show that A-to-I RNA editing has the capacity to tune protein function in response to external stimuli.

Original languageEnglish
Pages (from-to)1124-1134
Number of pages11
JournalNucleic Acids Research
Volume41
Issue number2
DOIs
Publication statusPublished - Jan 2013

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RNA Editing
Hippocampus
Alternative Splicing
AMPA Receptors
Proteins
Homeostasis

ASJC Scopus subject areas

  • Genetics

Cite this

Activity-regulated RNA editing in select neuronal subfields in hippocampus. / Balik, Ales; Penn, Andrew C.; Nemoda, Z.; Greger, Ingo H.

In: Nucleic Acids Research, Vol. 41, No. 2, 01.2013, p. 1124-1134.

Research output: Contribution to journalArticle

Balik, Ales ; Penn, Andrew C. ; Nemoda, Z. ; Greger, Ingo H. / Activity-regulated RNA editing in select neuronal subfields in hippocampus. In: Nucleic Acids Research. 2013 ; Vol. 41, No. 2. pp. 1124-1134.
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