Activity of oxytocinergic neurons in the supraoptic nucleus under stimulation of α2-adrenoceptors in brattleboro rats

Jana Bundzikova, Zdeno Pirnik, Jens D. Mikkelsen, D. Zelena, Alexander Kiss

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

Vasopressin (AVP) deficient homozygous Brattleboro rats exhibit severe osmotic challenges due to waterless chronic hypernatremia and hyperosmolality. We investigated the effect of xylazine, an α2-adrenoceptor agonist, on the activity of oxytocinergic (OXY) neurons in the supraoptic nucleus (SON) of homozygous (di/di), heterozygous (di/+), and control (+/+) rats. Ninety minutes after saline (0.1 mL/100 g b.w., i.p.) or xylazine injection (10 mg/kg, i.p.) rats were anaesthetized with pentobarbital (50 mg/kg i.p.) and sacrificed by transcardial perfusion with fixative. Activity of OXY neurons was evidenced by nuclear Fos protein immunoreactivity. Fos/OXY colabelings were analyzed on 40-μm thick coronal sections using computerized light microscope. As expected, plasma osmolality and water intake revealed high heterogenity within the di/di group of rats. Fos expression in SON of di/di rats was correlated with osmolality of each rat. In saline-treated rats, maximum activation of Fos reached around 4% in +/+, 20% in di/+ rats, and as much as 60% in di/di rats. Xylazine activated in SON about 70% of OXY-ergic neurons in +/+, 60% in di/+ rats, and more than 80% in di/di rats. The present findings indicate that in spite of the high spontaneous activity of SON OXY-ergic neurons due to the AVP deficiency in di/di rats, many of the silent OXY-ergic neurons in the SON remained acceptable for α2-adrenoceptor stimulation.

Original languageEnglish
Title of host publicationAnnals of the New York Academy of Sciences
Pages154-160
Number of pages7
Volume1148
DOIs
Publication statusPublished - Dec 2008

Publication series

NameAnnals of the New York Academy of Sciences
Volume1148
ISSN (Print)00778923
ISSN (Electronic)17496632

Fingerprint

Brattleboro Rats
Supraoptic Nucleus
Adrenergic Receptors
Neurons
Rats
Xylazine
Osmolar Concentration
Rat control
Hypernatremia
Fixatives
Pentobarbital
Nuclear Proteins
Vasopressins
Drinking
Microscopes

Keywords

  • Brattleboro rats
  • Fos-immunohistochemistry
  • Oxytocin
  • Supraoptic nucleus
  • Xylazine

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Bundzikova, J., Pirnik, Z., Mikkelsen, J. D., Zelena, D., & Kiss, A. (2008). Activity of oxytocinergic neurons in the supraoptic nucleus under stimulation of α2-adrenoceptors in brattleboro rats. In Annals of the New York Academy of Sciences (Vol. 1148, pp. 154-160). (Annals of the New York Academy of Sciences; Vol. 1148). https://doi.org/10.1196/annals.1410.016

Activity of oxytocinergic neurons in the supraoptic nucleus under stimulation of α2-adrenoceptors in brattleboro rats. / Bundzikova, Jana; Pirnik, Zdeno; Mikkelsen, Jens D.; Zelena, D.; Kiss, Alexander.

Annals of the New York Academy of Sciences. Vol. 1148 2008. p. 154-160 (Annals of the New York Academy of Sciences; Vol. 1148).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Bundzikova, J, Pirnik, Z, Mikkelsen, JD, Zelena, D & Kiss, A 2008, Activity of oxytocinergic neurons in the supraoptic nucleus under stimulation of α2-adrenoceptors in brattleboro rats. in Annals of the New York Academy of Sciences. vol. 1148, Annals of the New York Academy of Sciences, vol. 1148, pp. 154-160. https://doi.org/10.1196/annals.1410.016
Bundzikova J, Pirnik Z, Mikkelsen JD, Zelena D, Kiss A. Activity of oxytocinergic neurons in the supraoptic nucleus under stimulation of α2-adrenoceptors in brattleboro rats. In Annals of the New York Academy of Sciences. Vol. 1148. 2008. p. 154-160. (Annals of the New York Academy of Sciences). https://doi.org/10.1196/annals.1410.016
Bundzikova, Jana ; Pirnik, Zdeno ; Mikkelsen, Jens D. ; Zelena, D. ; Kiss, Alexander. / Activity of oxytocinergic neurons in the supraoptic nucleus under stimulation of α2-adrenoceptors in brattleboro rats. Annals of the New York Academy of Sciences. Vol. 1148 2008. pp. 154-160 (Annals of the New York Academy of Sciences).
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abstract = "Vasopressin (AVP) deficient homozygous Brattleboro rats exhibit severe osmotic challenges due to waterless chronic hypernatremia and hyperosmolality. We investigated the effect of xylazine, an α2-adrenoceptor agonist, on the activity of oxytocinergic (OXY) neurons in the supraoptic nucleus (SON) of homozygous (di/di), heterozygous (di/+), and control (+/+) rats. Ninety minutes after saline (0.1 mL/100 g b.w., i.p.) or xylazine injection (10 mg/kg, i.p.) rats were anaesthetized with pentobarbital (50 mg/kg i.p.) and sacrificed by transcardial perfusion with fixative. Activity of OXY neurons was evidenced by nuclear Fos protein immunoreactivity. Fos/OXY colabelings were analyzed on 40-μm thick coronal sections using computerized light microscope. As expected, plasma osmolality and water intake revealed high heterogenity within the di/di group of rats. Fos expression in SON of di/di rats was correlated with osmolality of each rat. In saline-treated rats, maximum activation of Fos reached around 4{\%} in +/+, 20{\%} in di/+ rats, and as much as 60{\%} in di/di rats. Xylazine activated in SON about 70{\%} of OXY-ergic neurons in +/+, 60{\%} in di/+ rats, and more than 80{\%} in di/di rats. The present findings indicate that in spite of the high spontaneous activity of SON OXY-ergic neurons due to the AVP deficiency in di/di rats, many of the silent OXY-ergic neurons in the SON remained acceptable for α2-adrenoceptor stimulation.",
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