Active water selective channels in the stomach: Investigation of aquaporins after ethanol and capsaicin treatment in rats

Beáta Bódis, Gergely Nagy, P. Németh, G. Mózsik

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Recent studies discovered the existence of aquaporins (AQP), suggesting their roles in the active, ATP dependent water secretion or absorption. Our recent development of the monoclonal antibody family against aquaporins (Type 1 and 4) allowed us a good opportunity to investigate the mechanism of the gastric mucosal edema in a rat model. The aim of our study was to evaluate the changes in the tissue level of aquaporins (AQP1 and AQP4) after ethanol and capsaicin treatment in rat stomach. Materials and methods: the experiments were carried out on Sprague-Dawley rats weighing 150-200 g. The animals were fasted for 24 h, after the 1 ml of ethanol (50% v/v) or capsaicin (2 mg/ml) was given intragastrically. Rats were sacrificed after 5, 30, 60, 120 and 240 min, the tissue level of AQP1 and AQP4 was investigated immunoserologically by ELISA and dot-blot methods using our monoclonal antibodies. The location of these aquaporins in the gastric tissue was demonstrated by immunohistochemistry. Results: (1) in ethanol-treated stomach, both AQP1 and AQP4 increased after 5 min simultaneously with gastritis, then decreased dramatically depending on time. (2) In the capsaicin-treated group there were no changes in the tissue level of aquaporins in the first hour. After 60 min both AQP1 and AQP4 increased in the stomach without any macroscopically detectable changes, then decreased depending on time. (3) The immunohistochemical investigations using our monoclonal antibodies seem to support our present quantitative results. Conclusion: chemically induced gastric mucosal lesions are started by an extended edema. In the induction of the edema and the subsequential gastric injury, aquaporins (both AQP1 and AQP4) play an important role in the maintenance of mucosal integrity.

Original languageEnglish
Pages (from-to)271-275
Number of pages5
JournalJournal of Physiology Paris
Volume95
Issue number1-6
DOIs
Publication statusPublished - 2001

Fingerprint

Aquaporins
Capsaicin
Stomach
Ethanol
Edema
Monoclonal Antibodies
Therapeutics
Aquaporin 1
Aquaporin 4
Gastritis
Sprague Dawley Rats
Adenosine Triphosphate
Enzyme-Linked Immunosorbent Assay
Immunohistochemistry
Maintenance
Water
Wounds and Injuries

Keywords

  • Aquaporin
  • Capsaicin
  • Cytoprotection
  • Edema
  • Ethanol
  • Mucosal integrity

ASJC Scopus subject areas

  • Neuroscience(all)
  • Physiology (medical)

Cite this

Active water selective channels in the stomach : Investigation of aquaporins after ethanol and capsaicin treatment in rats. / Bódis, Beáta; Nagy, Gergely; Németh, P.; Mózsik, G.

In: Journal of Physiology Paris, Vol. 95, No. 1-6, 2001, p. 271-275.

Research output: Contribution to journalArticle

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abstract = "Recent studies discovered the existence of aquaporins (AQP), suggesting their roles in the active, ATP dependent water secretion or absorption. Our recent development of the monoclonal antibody family against aquaporins (Type 1 and 4) allowed us a good opportunity to investigate the mechanism of the gastric mucosal edema in a rat model. The aim of our study was to evaluate the changes in the tissue level of aquaporins (AQP1 and AQP4) after ethanol and capsaicin treatment in rat stomach. Materials and methods: the experiments were carried out on Sprague-Dawley rats weighing 150-200 g. The animals were fasted for 24 h, after the 1 ml of ethanol (50{\%} v/v) or capsaicin (2 mg/ml) was given intragastrically. Rats were sacrificed after 5, 30, 60, 120 and 240 min, the tissue level of AQP1 and AQP4 was investigated immunoserologically by ELISA and dot-blot methods using our monoclonal antibodies. The location of these aquaporins in the gastric tissue was demonstrated by immunohistochemistry. Results: (1) in ethanol-treated stomach, both AQP1 and AQP4 increased after 5 min simultaneously with gastritis, then decreased dramatically depending on time. (2) In the capsaicin-treated group there were no changes in the tissue level of aquaporins in the first hour. After 60 min both AQP1 and AQP4 increased in the stomach without any macroscopically detectable changes, then decreased depending on time. (3) The immunohistochemical investigations using our monoclonal antibodies seem to support our present quantitative results. Conclusion: chemically induced gastric mucosal lesions are started by an extended edema. In the induction of the edema and the subsequential gastric injury, aquaporins (both AQP1 and AQP4) play an important role in the maintenance of mucosal integrity.",
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