Active-site residues in the type IV prepilin peptidase homologue PibD from the archaeon Sulfolobus solfataricus

Z. Szabó, Sonja Verena Albers, Arnold J M Driessen

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Archaeal preflagellin peptidases and bacterial type IV prepilin peptidases belong to a family of aspartic acid proteases that cleave the leader peptides of precursor proteins with type IV prepilin signal sequences. The substrate repertoire of PibD from the crenarchaeon Sulfolobus solfataricus is unusually diverse. In addition to flagellin, PibD cleaves three sugar-binding proteins unique to this species and a number of proteins with unknown function. Here we demonstrate that PibD contains two aspartic acid residues that are essential for cleavage activity. An additional pair of aspartic acids in a large cytoplasmic loop is also important for function and is possibly involved in leader peptide recognition. Combining the results of transmembrane segment predictions and cysteine-labeling experiments, we suggest a membrane topology model for PibD with the active-site aspartic acid residues exposed to the cytosol.

Original languageEnglish
Pages (from-to)1437-1443
Number of pages7
JournalJournal of Bacteriology
Volume188
Issue number4
DOIs
Publication statusPublished - Feb 2006

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Sulfolobus solfataricus
Archaea
Protein Sorting Signals
Aspartic Acid
Catalytic Domain
Aspartic Acid Proteases
Flagellin
Protein Precursors
Cytosol
Cysteine
Carrier Proteins
Membranes
type IV prepilin peptidase
Proteins

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Active-site residues in the type IV prepilin peptidase homologue PibD from the archaeon Sulfolobus solfataricus. / Szabó, Z.; Albers, Sonja Verena; Driessen, Arnold J M.

In: Journal of Bacteriology, Vol. 188, No. 4, 02.2006, p. 1437-1443.

Research output: Contribution to journalArticle

Szabó, Z. ; Albers, Sonja Verena ; Driessen, Arnold J M. / Active-site residues in the type IV prepilin peptidase homologue PibD from the archaeon Sulfolobus solfataricus. In: Journal of Bacteriology. 2006 ; Vol. 188, No. 4. pp. 1437-1443.
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