Active site distortion is sufficient for proteinase inhibition by serpins: Structure of the covalent complex of α1-proteinase inhibitor with porcine pancreatic elastase

Alexey Dementiev, J. Dobó, Peter G W Gettins

Research output: Contribution to journalArticle

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Abstract

We report here the x-ray structure of a covalent serpin-proteinase complex, α1-proteinase inhibitor (α1PI) with porcine pancreatic elastase (PPE), which differs from the only other x-ray structure of such a complex, that of α1PI with trypsin, in showing nearly complete definition of the proteinase. α1PI complexes with trypsin, PPE, and human neutrophil elastase (HNE) showed similar rates of deacylation and enhanced susceptibility to proteolysis by exogenous proteinases in solution. The differences between the two x-ray structures therefore cannot arise from intrinsic differences in the inhibition mechanism. However, self-proteolysis of purified complex resulted in rapid cleavage of the trypsin complex, slower cleavage of the PPE complex, and only minimal cleavage of the HNE complex. This suggests that the earlier α1PI-trypsin complex may have been proteolyzed and that the present structure is more likely to be representative of serpin-proteinase complexes. The present structure shows that active site distortion alone is sufficient for inhibition and suggests that enhanced proteolysis is not necessarily exploited in vivo.

Original languageEnglish
Pages (from-to)3452-3457
Number of pages6
JournalJournal of Biological Chemistry
Volume281
Issue number6
DOIs
Publication statusPublished - Feb 10 2006

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Serpins
Pancreatic Elastase
Catalytic Domain
Peptide Hydrolases
Swine
Proteolysis
Trypsin
Enzyme inhibition
Leukocyte Elastase
Trypsin Inhibitors
X-Rays
X rays

ASJC Scopus subject areas

  • Biochemistry

Cite this

Active site distortion is sufficient for proteinase inhibition by serpins : Structure of the covalent complex of α1-proteinase inhibitor with porcine pancreatic elastase. / Dementiev, Alexey; Dobó, J.; Gettins, Peter G W.

In: Journal of Biological Chemistry, Vol. 281, No. 6, 10.02.2006, p. 3452-3457.

Research output: Contribution to journalArticle

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