Active cell and ECM movements during development

Anastasiia Aleksandrova, Brenda J. Rongish, Charles D. Little, András Czirók

Research output: Chapter in Book/Report/Conference proceedingChapter

5 Citations (Scopus)


Dynamic imaging of the extracellular matrix (ECM) and cells can reveal how tissues are formed. Displacement differences between cells and the adjacent ECM scaffold can be used to establish active movements of mesenchymal cells. Cells can also generate large-scale tissue movements in which cell and ECM displacements are shared.We describe computational methods for analyzing multi-spectral time-lapse image sequences. The resulting data can distinguish between local "active" cellular motion versus largescale, tissue movements, both of which occur during organogenesis. The movement data also provide the basis for construction of realistic biomechanical models and computer simulations of in vivo tissue formation.

Original languageEnglish
Title of host publicationTissue Morphogenesis: Methods and Protocols
PublisherSpringer New York
Number of pages10
ISBN (Print)9781493911646, 9781493911639
Publication statusPublished - Sep 22 2014



  • Avian development
  • Cell motility
  • Computational analysis
  • Time-lapse imaging

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Aleksandrova, A., Rongish, B. J., Little, C. D., & Czirók, A. (2014). Active cell and ECM movements during development. In Tissue Morphogenesis: Methods and Protocols (pp. 123-132). Springer New York.