Activation volume and activation energy of the protochlorophyllide phototransformation and the Shibata shift determined with high pressure fluorescence spectroscopy

Research output: Contribution to journalArticle

Abstract

The kinetics of protochlorophyllide phototransformation and of the subsequent blue shift were studied in homogenates prepared from etiolated wheat leaves using fluorescence spectroscopy at high pressures. The pressure dependence of processes was studied between 0.1 and 400 MPa. The activation volumes were calculated from the pressure dependence of the kinetical curves of the processes and were compared at 20°C and at 40°C. The temperature dependence of the processes was analyzed at atmospheric pressure (0.1 MPa) between 10° and 40°C. The activation energy of the processes at atmospheric pressure was calculated using Arrhenius plot. The simultaneous variation/change of temperature and pressure provides information about the molecular events occuring during the activation of the processes.

Original languageEnglish
Pages (from-to)151-153
Number of pages3
JournalActa Biologica Szegediensis
Volume46
Issue number3-4
Publication statusPublished - 2002

Fingerprint

fluorescence emission spectroscopy
Protochlorophyllide
protochlorophyllides
Fluorescence Spectrometry
Fluorescence spectroscopy
activation energy
Activation energy
Chemical activation
Pressure
Atmospheric Pressure
atmospheric pressure
Atmospheric pressure
Arrhenius plots
Temperature
Triticum
temperature
kinetics
wheat
Kinetics
leaves

Keywords

  • Activation energy
  • Avtivation volume
  • Chlorophyllide
  • High pressure fluorescence
  • Protochlorophyllide
  • Shibata shift
  • Spectroscopy

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Cell Biology
  • Applied Microbiology and Biotechnology
  • Neuroscience(all)

Cite this

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abstract = "The kinetics of protochlorophyllide phototransformation and of the subsequent blue shift were studied in homogenates prepared from etiolated wheat leaves using fluorescence spectroscopy at high pressures. The pressure dependence of processes was studied between 0.1 and 400 MPa. The activation volumes were calculated from the pressure dependence of the kinetical curves of the processes and were compared at 20°C and at 40°C. The temperature dependence of the processes was analyzed at atmospheric pressure (0.1 MPa) between 10° and 40°C. The activation energy of the processes at atmospheric pressure was calculated using Arrhenius plot. The simultaneous variation/change of temperature and pressure provides information about the molecular events occuring during the activation of the processes.",
keywords = "Activation energy, Avtivation volume, Chlorophyllide, High pressure fluorescence, Protochlorophyllide, Shibata shift, Spectroscopy",
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journal = "Acta Biologica Szegediensis",
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TY - JOUR

T1 - Activation volume and activation energy of the protochlorophyllide phototransformation and the Shibata shift determined with high pressure fluorescence spectroscopy

AU - Solymosi, K.

AU - Smeller, L.

AU - Fidy, J.

AU - Böddi, B.

PY - 2002

Y1 - 2002

N2 - The kinetics of protochlorophyllide phototransformation and of the subsequent blue shift were studied in homogenates prepared from etiolated wheat leaves using fluorescence spectroscopy at high pressures. The pressure dependence of processes was studied between 0.1 and 400 MPa. The activation volumes were calculated from the pressure dependence of the kinetical curves of the processes and were compared at 20°C and at 40°C. The temperature dependence of the processes was analyzed at atmospheric pressure (0.1 MPa) between 10° and 40°C. The activation energy of the processes at atmospheric pressure was calculated using Arrhenius plot. The simultaneous variation/change of temperature and pressure provides information about the molecular events occuring during the activation of the processes.

AB - The kinetics of protochlorophyllide phototransformation and of the subsequent blue shift were studied in homogenates prepared from etiolated wheat leaves using fluorescence spectroscopy at high pressures. The pressure dependence of processes was studied between 0.1 and 400 MPa. The activation volumes were calculated from the pressure dependence of the kinetical curves of the processes and were compared at 20°C and at 40°C. The temperature dependence of the processes was analyzed at atmospheric pressure (0.1 MPa) between 10° and 40°C. The activation energy of the processes at atmospheric pressure was calculated using Arrhenius plot. The simultaneous variation/change of temperature and pressure provides information about the molecular events occuring during the activation of the processes.

KW - Activation energy

KW - Avtivation volume

KW - Chlorophyllide

KW - High pressure fluorescence

KW - Protochlorophyllide

KW - Shibata shift

KW - Spectroscopy

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