Activation of the miR-17 family and miR-21 during murine kidney ischemia-reperfusion injury

Tamás Kaucsár, Csaba Révész, Mária Godó, T. Krenács, Mihály Albert, Csaba Imre Szalay, L. Rosivall, Zoltán Benyó, Sándor Bátkai, Thomas Thum, G. Szénási, P. Hamar

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Background: Ischemia-reperfusion (I/R) is the main cause of acute kidney injury (AKI) in patients. We investigated renal microRNA (miRNA) expression profiles and the time course of changes in selected miRNA expressions after renal I/R to characterize the miRNA network activated during development and recovery from AKI. Methods and Results: One day after lethal (30 minutes) and sublethal (20 minutes) renal ischemia, AKI was verified by renal histology (tubular necrosis, regeneration), blood urea nitrogen (BUN) level, renal mRNA expression, and plasma concentration of neutrophil gelatinase-associated lipocalin (NGAL) in C57BL/6J mice. On the first day after 30-minute, lethal I/R miR-21, miR-17-5p, and miR-106a were elevated out of the 21 miRNAs successfully profiled on the Luminex multiplex assay. After 20-minute, sublethal I/R, renal miR-17-5p and miR-106a expressions were elevated on the first and second days of reperfusion, while miR-21 expression increased later and lasted longer. Renal miR-17-5p and miR-21 expressions correlated with each other. Renal function returned to normal on the fourth day after sublethal I/R. Conclusions: Our results demonstrate that besides miR-21, miR-17-5p, and miR-106a are additionally activated during the maintenance and recovery phases of renal I/R injury. Furthermore, a correlation between renal miR-17-5p and miR-21 expressions warrants further investigation of how they may influence each other and the outcome of renal ischemia-reperfusion injury.

Original languageEnglish
Pages (from-to)344-354
Number of pages11
JournalNucleic Acid Therapeutics
Volume23
Issue number5
DOIs
Publication statusPublished - Oct 1 2013

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Reperfusion Injury
MicroRNAs
Chemical activation
Kidney
Reperfusion
Ischemia
Lipocalins
Recovery
Gelatinases
Histology
Acute Kidney Injury
Urea
Assays
Blood
Nitrogen
Plasmas
Messenger RNA
Blood Urea Nitrogen
Inbred C57BL Mouse
Regeneration

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Molecular Medicine
  • Biochemistry
  • Drug Discovery

Cite this

Activation of the miR-17 family and miR-21 during murine kidney ischemia-reperfusion injury. / Kaucsár, Tamás; Révész, Csaba; Godó, Mária; Krenács, T.; Albert, Mihály; Szalay, Csaba Imre; Rosivall, L.; Benyó, Zoltán; Bátkai, Sándor; Thum, Thomas; Szénási, G.; Hamar, P.

In: Nucleic Acid Therapeutics, Vol. 23, No. 5, 01.10.2013, p. 344-354.

Research output: Contribution to journalArticle

Kaucsár, Tamás ; Révész, Csaba ; Godó, Mária ; Krenács, T. ; Albert, Mihály ; Szalay, Csaba Imre ; Rosivall, L. ; Benyó, Zoltán ; Bátkai, Sándor ; Thum, Thomas ; Szénási, G. ; Hamar, P. / Activation of the miR-17 family and miR-21 during murine kidney ischemia-reperfusion injury. In: Nucleic Acid Therapeutics. 2013 ; Vol. 23, No. 5. pp. 344-354.
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abstract = "Background: Ischemia-reperfusion (I/R) is the main cause of acute kidney injury (AKI) in patients. We investigated renal microRNA (miRNA) expression profiles and the time course of changes in selected miRNA expressions after renal I/R to characterize the miRNA network activated during development and recovery from AKI. Methods and Results: One day after lethal (30 minutes) and sublethal (20 minutes) renal ischemia, AKI was verified by renal histology (tubular necrosis, regeneration), blood urea nitrogen (BUN) level, renal mRNA expression, and plasma concentration of neutrophil gelatinase-associated lipocalin (NGAL) in C57BL/6J mice. On the first day after 30-minute, lethal I/R miR-21, miR-17-5p, and miR-106a were elevated out of the 21 miRNAs successfully profiled on the Luminex multiplex assay. After 20-minute, sublethal I/R, renal miR-17-5p and miR-106a expressions were elevated on the first and second days of reperfusion, while miR-21 expression increased later and lasted longer. Renal miR-17-5p and miR-21 expressions correlated with each other. Renal function returned to normal on the fourth day after sublethal I/R. Conclusions: Our results demonstrate that besides miR-21, miR-17-5p, and miR-106a are additionally activated during the maintenance and recovery phases of renal I/R injury. Furthermore, a correlation between renal miR-17-5p and miR-21 expressions warrants further investigation of how they may influence each other and the outcome of renal ischemia-reperfusion injury.",
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T1 - Activation of the miR-17 family and miR-21 during murine kidney ischemia-reperfusion injury

AU - Kaucsár, Tamás

AU - Révész, Csaba

AU - Godó, Mária

AU - Krenács, T.

AU - Albert, Mihály

AU - Szalay, Csaba Imre

AU - Rosivall, L.

AU - Benyó, Zoltán

AU - Bátkai, Sándor

AU - Thum, Thomas

AU - Szénási, G.

AU - Hamar, P.

PY - 2013/10/1

Y1 - 2013/10/1

N2 - Background: Ischemia-reperfusion (I/R) is the main cause of acute kidney injury (AKI) in patients. We investigated renal microRNA (miRNA) expression profiles and the time course of changes in selected miRNA expressions after renal I/R to characterize the miRNA network activated during development and recovery from AKI. Methods and Results: One day after lethal (30 minutes) and sublethal (20 minutes) renal ischemia, AKI was verified by renal histology (tubular necrosis, regeneration), blood urea nitrogen (BUN) level, renal mRNA expression, and plasma concentration of neutrophil gelatinase-associated lipocalin (NGAL) in C57BL/6J mice. On the first day after 30-minute, lethal I/R miR-21, miR-17-5p, and miR-106a were elevated out of the 21 miRNAs successfully profiled on the Luminex multiplex assay. After 20-minute, sublethal I/R, renal miR-17-5p and miR-106a expressions were elevated on the first and second days of reperfusion, while miR-21 expression increased later and lasted longer. Renal miR-17-5p and miR-21 expressions correlated with each other. Renal function returned to normal on the fourth day after sublethal I/R. Conclusions: Our results demonstrate that besides miR-21, miR-17-5p, and miR-106a are additionally activated during the maintenance and recovery phases of renal I/R injury. Furthermore, a correlation between renal miR-17-5p and miR-21 expressions warrants further investigation of how they may influence each other and the outcome of renal ischemia-reperfusion injury.

AB - Background: Ischemia-reperfusion (I/R) is the main cause of acute kidney injury (AKI) in patients. We investigated renal microRNA (miRNA) expression profiles and the time course of changes in selected miRNA expressions after renal I/R to characterize the miRNA network activated during development and recovery from AKI. Methods and Results: One day after lethal (30 minutes) and sublethal (20 minutes) renal ischemia, AKI was verified by renal histology (tubular necrosis, regeneration), blood urea nitrogen (BUN) level, renal mRNA expression, and plasma concentration of neutrophil gelatinase-associated lipocalin (NGAL) in C57BL/6J mice. On the first day after 30-minute, lethal I/R miR-21, miR-17-5p, and miR-106a were elevated out of the 21 miRNAs successfully profiled on the Luminex multiplex assay. After 20-minute, sublethal I/R, renal miR-17-5p and miR-106a expressions were elevated on the first and second days of reperfusion, while miR-21 expression increased later and lasted longer. Renal miR-17-5p and miR-21 expressions correlated with each other. Renal function returned to normal on the fourth day after sublethal I/R. Conclusions: Our results demonstrate that besides miR-21, miR-17-5p, and miR-106a are additionally activated during the maintenance and recovery phases of renal I/R injury. Furthermore, a correlation between renal miR-17-5p and miR-21 expressions warrants further investigation of how they may influence each other and the outcome of renal ischemia-reperfusion injury.

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