Activation of the lipoxygenase pathway in the methionine enkephalin induced respiratory burst in human polymorphonuclear leukocytes

J. T. Nagy, G. Foris, T. Fulop, G. Paragh, N. P. Plotnikoff

Research output: Contribution to journalArticle

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Abstract

In comparative studies of f-met-Leu-Phe (FMLP) and methionine enkephalin (ME) induced polymorphonuclear leukocyte (PMNL) stimulation the following results were obtained: (i) both FMLP and ME increased the intracellular killing (IK) capability of human PMNLs probably through NADPH oxidase activation, (ii) the ME-induced respiratory burst (RB) differed from the chemotactic peptide FMLP-triggered superoxide generation because the former was not accompanied by the activation of the glutathione system and the duration of the superoxide production was prolonged. The reaction was dependent on lipoxygenation, was potentiated by indomethacin (IM) and was inhibited by nordihidro-guairetic acid (NDGA), (iii) both 14C-arachidonic acid (14C-AA) release and leukotriene B4 (LTB4) synthesis of ME-treated PMNLs were elevated as compared to those of FMLP triggered cells. Our results suggest that lipoxygenation and even an increased LTB4 synthesis are involved in the ME-induced RB of leukocytes.

Original languageEnglish
Pages (from-to)2299-2306
Number of pages8
JournalLife Sciences
Volume42
Issue number22
DOIs
Publication statusPublished - 1988

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Methionine Enkephalin
Lipoxygenase
Respiratory Burst
Neutrophils
Chemical activation
methionyl-leucyl-phenylalanine
Leucine Enkephalin
Leukotriene B4
Superoxides
NADPH Oxidase
Arachidonic Acid
Indomethacin
Glutathione
Leukocytes
Peptides
Acids

ASJC Scopus subject areas

  • Pharmacology

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Activation of the lipoxygenase pathway in the methionine enkephalin induced respiratory burst in human polymorphonuclear leukocytes. / Nagy, J. T.; Foris, G.; Fulop, T.; Paragh, G.; Plotnikoff, N. P.

In: Life Sciences, Vol. 42, No. 22, 1988, p. 2299-2306.

Research output: Contribution to journalArticle

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AU - Foris, G.

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AU - Paragh, G.

AU - Plotnikoff, N. P.

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AB - In comparative studies of f-met-Leu-Phe (FMLP) and methionine enkephalin (ME) induced polymorphonuclear leukocyte (PMNL) stimulation the following results were obtained: (i) both FMLP and ME increased the intracellular killing (IK) capability of human PMNLs probably through NADPH oxidase activation, (ii) the ME-induced respiratory burst (RB) differed from the chemotactic peptide FMLP-triggered superoxide generation because the former was not accompanied by the activation of the glutathione system and the duration of the superoxide production was prolonged. The reaction was dependent on lipoxygenation, was potentiated by indomethacin (IM) and was inhibited by nordihidro-guairetic acid (NDGA), (iii) both 14C-arachidonic acid (14C-AA) release and leukotriene B4 (LTB4) synthesis of ME-treated PMNLs were elevated as compared to those of FMLP triggered cells. Our results suggest that lipoxygenation and even an increased LTB4 synthesis are involved in the ME-induced RB of leukocytes.

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