Activation of the Drosophila NF-κB factor Relish by rapid endoproteolytic cleavage

Svenja Stöven, I. Andó, Latha Kadalayil, Ylva Engström, Dan Hultmark

Research output: Contribution to journalArticle

208 Citations (Scopus)

Abstract

The Rel/NF-κB transcription factor Relish plays a key role in the humoral immune response in Drosophila. We now find that activation of this innate immune response is preceded by rapid proteolytic cleavage of Relish into two parts. An N-terminal fragment, containing the DNA-binding Rel homology domain, translocates to the nucleus where it binds to the promoter of the Cecropin A1 gene and probably to the promoters of other antimicrobial peptide genes. The C-terminal IκB-like fragment remains in the cytoplasm. This endoproteolytic cleavage does not involve the proteasome, requires the DREDD caspase, and is different from previously described mechanisms for Rel factor activation.

Original languageEnglish
Pages (from-to)347-352
Number of pages6
JournalEMBO Reports
Volume1
Issue number4
Publication statusPublished - 2000

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Drosophila
Cecropins
Genes
Chemical activation
Proteasome Endopeptidase Complex
Humoral Immunity
Caspases
Innate Immunity
Cytoplasm
Transcription Factors
Peptides
DNA
3'-(1-butylphosphoryl)adenosine

ASJC Scopus subject areas

  • Cell Biology
  • Genetics

Cite this

Stöven, S., Andó, I., Kadalayil, L., Engström, Y., & Hultmark, D. (2000). Activation of the Drosophila NF-κB factor Relish by rapid endoproteolytic cleavage. EMBO Reports, 1(4), 347-352.

Activation of the Drosophila NF-κB factor Relish by rapid endoproteolytic cleavage. / Stöven, Svenja; Andó, I.; Kadalayil, Latha; Engström, Ylva; Hultmark, Dan.

In: EMBO Reports, Vol. 1, No. 4, 2000, p. 347-352.

Research output: Contribution to journalArticle

Stöven, S, Andó, I, Kadalayil, L, Engström, Y & Hultmark, D 2000, 'Activation of the Drosophila NF-κB factor Relish by rapid endoproteolytic cleavage', EMBO Reports, vol. 1, no. 4, pp. 347-352.
Stöven S, Andó I, Kadalayil L, Engström Y, Hultmark D. Activation of the Drosophila NF-κB factor Relish by rapid endoproteolytic cleavage. EMBO Reports. 2000;1(4):347-352.
Stöven, Svenja ; Andó, I. ; Kadalayil, Latha ; Engström, Ylva ; Hultmark, Dan. / Activation of the Drosophila NF-κB factor Relish by rapid endoproteolytic cleavage. In: EMBO Reports. 2000 ; Vol. 1, No. 4. pp. 347-352.
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