Activation of sodium-proton exchange is not a prerequisite for Ca2+ mobilization and aggregation in human platelets

L. Hunyady, B. Sarkadi, Edward J. Cragoe, A. Spät, George Gárdos

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Recently it has been suggested [(1987) Nature 325, 456-458; (1987) FEBS Lett. 212, 123-126] that the activation of Na+/H+ exchange is a prerequisite for platelet aggregation and the development of the Ca2+ signal. As direct evidence for the role of the Na+/H+-exchange pathway the inhibition of the Ca2+ signal by EIPA, a specific inhibitor of Na+/H+ exchange, was offered. Here we demonstrate that low concentrations of EIPA (below 1 μM) completely block Na+/H+ exchange while EIPA inhibits aggregation or Ca2+ mobilization only in concentrations 100-times greater than 1 μM. Moreover, another amiloride analogue, CBDMB, developed to act predominantly on Na+/Ca2+ exchange, does not affect Na+/H+ exchange in platelets but blocks aggregation and Ca2+ mobilization. We conclude that while Na+/H+ exchange has a fundamental role in platelet functions it is not prerequisite for the development of Ca2+ signal and aggregation.

Original languageEnglish
Pages (from-to)72-76
Number of pages5
JournalFEBS Letters
Volume225
Issue number1-2
DOIs
Publication statusPublished - Dec 10 1987

Fingerprint

Platelets
Protons
Ion exchange
Blood Platelets
Agglomeration
Sodium
Chemical activation
Platelet Aggregation
Amiloride
ethylisopropylamiloride

Keywords

  • (Human platelet)
  • Amiloride analogue
  • Ca signal
  • Na/H exchange
  • Thrombin

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Activation of sodium-proton exchange is not a prerequisite for Ca2+ mobilization and aggregation in human platelets. / Hunyady, L.; Sarkadi, B.; Cragoe, Edward J.; Spät, A.; Gárdos, George.

In: FEBS Letters, Vol. 225, No. 1-2, 10.12.1987, p. 72-76.

Research output: Contribution to journalArticle

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