Activation of lymphocytes alters Fc receptor-β2-microglobulin interrelationship on the lymphocyte surface

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Abstract

The effect of anti-β2-microglobulin (anti-B2Mi) on the expression of Fc receptors (FcR) of human lymphocytes was compared on resting and activated cells. Previously we reported that anti-B2Mi induces a "co-shedding" of FcR with the β2-microglobulin (B2Mi)-anti-B2Mi complexes when used under the conditions where the redistribution of membrane molecules is allowed (Sármay et al., Cell. Immunol.56, 452, 1980; Sármay et al. Immunology36, 339, 1979). Furthermore our group also described two types of FcR-bearing cells, one which shed their FcR during a temperature shift from 4 to 37 °C (FcRI+ cells) and the other which has an immobile type FcR under the same circumstances (FcRII+ cells) (Sàndor et al., Immunology38, 553, 1979; Sármay et al., Immunology34, 315, 1978). In this work we have characterized the FcR released from the membrane as a consequence of anti-B2Mi treatment. We have found that they are the mobile, FcRI type. It was proved that the shedding of this FcRI is a consequence of the anti-B2MI-induced transformation of FcRII into the FcRI form on the membrane of the antibody-treated lymphocytes. On the activated T cells, however, anti-B2Mi is incapable of inducing the same phenomenon in the early phase of activation. In contrast, FcR expression is blocked by anti-B2Mi treatment similarly to that on resting lymphocytes, on the surface of activated B cells, or on activated T cells in the later phases of activation.

Original languageEnglish
Pages (from-to)73-82
Number of pages10
JournalCellular Immunology
Volume78
Issue number1
DOIs
Publication statusPublished - 1983

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Fc Receptors
Lymphocyte Activation
Lymphocytes
Membranes
T-Lymphocytes
B-Lymphocytes
Temperature
Antibodies

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Cite this

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title = "Activation of lymphocytes alters Fc receptor-β2-microglobulin interrelationship on the lymphocyte surface",
abstract = "The effect of anti-β2-microglobulin (anti-B2Mi) on the expression of Fc receptors (FcR) of human lymphocytes was compared on resting and activated cells. Previously we reported that anti-B2Mi induces a {"}co-shedding{"} of FcR with the β2-microglobulin (B2Mi)-anti-B2Mi complexes when used under the conditions where the redistribution of membrane molecules is allowed (S{\'a}rmay et al., Cell. Immunol.56, 452, 1980; S{\'a}rmay et al. Immunology36, 339, 1979). Furthermore our group also described two types of FcR-bearing cells, one which shed their FcR during a temperature shift from 4 to 37 °C (FcRI+ cells) and the other which has an immobile type FcR under the same circumstances (FcRII+ cells) (S{\`a}ndor et al., Immunology38, 553, 1979; S{\'a}rmay et al., Immunology34, 315, 1978). In this work we have characterized the FcR released from the membrane as a consequence of anti-B2Mi treatment. We have found that they are the mobile, FcRI type. It was proved that the shedding of this FcRI is a consequence of the anti-B2MI-induced transformation of FcRII into the FcRI form on the membrane of the antibody-treated lymphocytes. On the activated T cells, however, anti-B2Mi is incapable of inducing the same phenomenon in the early phase of activation. In contrast, FcR expression is blocked by anti-B2Mi treatment similarly to that on resting lymphocytes, on the surface of activated B cells, or on activated T cells in the later phases of activation.",
author = "G. S{\'a}rmay and J. Gergely",
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T1 - Activation of lymphocytes alters Fc receptor-β2-microglobulin interrelationship on the lymphocyte surface

AU - Sármay, G.

AU - Gergely, J.

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N2 - The effect of anti-β2-microglobulin (anti-B2Mi) on the expression of Fc receptors (FcR) of human lymphocytes was compared on resting and activated cells. Previously we reported that anti-B2Mi induces a "co-shedding" of FcR with the β2-microglobulin (B2Mi)-anti-B2Mi complexes when used under the conditions where the redistribution of membrane molecules is allowed (Sármay et al., Cell. Immunol.56, 452, 1980; Sármay et al. Immunology36, 339, 1979). Furthermore our group also described two types of FcR-bearing cells, one which shed their FcR during a temperature shift from 4 to 37 °C (FcRI+ cells) and the other which has an immobile type FcR under the same circumstances (FcRII+ cells) (Sàndor et al., Immunology38, 553, 1979; Sármay et al., Immunology34, 315, 1978). In this work we have characterized the FcR released from the membrane as a consequence of anti-B2Mi treatment. We have found that they are the mobile, FcRI type. It was proved that the shedding of this FcRI is a consequence of the anti-B2MI-induced transformation of FcRII into the FcRI form on the membrane of the antibody-treated lymphocytes. On the activated T cells, however, anti-B2Mi is incapable of inducing the same phenomenon in the early phase of activation. In contrast, FcR expression is blocked by anti-B2Mi treatment similarly to that on resting lymphocytes, on the surface of activated B cells, or on activated T cells in the later phases of activation.

AB - The effect of anti-β2-microglobulin (anti-B2Mi) on the expression of Fc receptors (FcR) of human lymphocytes was compared on resting and activated cells. Previously we reported that anti-B2Mi induces a "co-shedding" of FcR with the β2-microglobulin (B2Mi)-anti-B2Mi complexes when used under the conditions where the redistribution of membrane molecules is allowed (Sármay et al., Cell. Immunol.56, 452, 1980; Sármay et al. Immunology36, 339, 1979). Furthermore our group also described two types of FcR-bearing cells, one which shed their FcR during a temperature shift from 4 to 37 °C (FcRI+ cells) and the other which has an immobile type FcR under the same circumstances (FcRII+ cells) (Sàndor et al., Immunology38, 553, 1979; Sármay et al., Immunology34, 315, 1978). In this work we have characterized the FcR released from the membrane as a consequence of anti-B2Mi treatment. We have found that they are the mobile, FcRI type. It was proved that the shedding of this FcRI is a consequence of the anti-B2MI-induced transformation of FcRII into the FcRI form on the membrane of the antibody-treated lymphocytes. On the activated T cells, however, anti-B2Mi is incapable of inducing the same phenomenon in the early phase of activation. In contrast, FcR expression is blocked by anti-B2Mi treatment similarly to that on resting lymphocytes, on the surface of activated B cells, or on activated T cells in the later phases of activation.

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