Activation of latent RNAase by antibodies against rat liver RNAase inhibitor

Hitomi Yasuna, Sataro Goto

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

RNAase inhibitor was purified to homogeneity from rat liver. The purified inhibitor was identified by measuring the activity in gel slices after polyacrylamide gel electrophoresis under non-denaturing conditions. Antibodies were prepared by immunization of guinea pigs with the inhibitor. The antibodies formed single precipitin lines with free RNAase inhibitor and RNAase A-inhibitor complex that fused in a micro-Ouchterlony test. Partially purified IgG from immunized animals, but not control animals, inhibited the activities of the inhibitor and the inhibitor in the complex. In the latter case, the RNAase-inhibitor complex was dissociated and active RNAase was liberated.

Original languageEnglish
Pages (from-to)220-226
Number of pages7
JournalBiochimica et Biophysica Acta (BBA)/Protein Structure and Molecular
Volume874
Issue number2
DOIs
Publication statusPublished - Nov 21 1986

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Keywords

  • (Rat liver)
  • RNA degradation
  • RNAase inhibitor
  • latent RNAase

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology

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