Activation of human T lymphocytes via the CD2 antigen results in tyrosine phosphorylation of T cell antigen receptor ζ-chains

E. Monostori, Dev Desai, Marion H. Brown, Doreen A. Cantrell, Michael J. Crumpton

Research output: Contribution to journalArticle

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Abstract

The phosphorylation of the invariant chains associated with the human TCR has been investigated after the stimulation of T lymphocytes with CD2 mAb T112 and T113, PHA, or phorbol 12,13-dibutyrate. As described previously, stimulation of T cells with either CD2 mAb or phorbol 12,13-dibutyrate resulted in the phosphorylation of the CD3 γ-chain. The combination of T112 and T113 mAb also induced phosphorylation of the TCR ζ-chain. The phosphorylated ζ-polypeptide of CD2-activated cells was immunoprecipitated with antiphosphotyrosine antibodies and migrated to a 21- to 23-kDa position during SDS/PAGE. These results indicate that stimulation of human T cells via the CD2 Ag with the T112 and T113 mAb activates not only protein kinase C but also tyrosine kinase(s), resulting in the phosphorylation of the CD3 γ-chain and the tyrosine phosphorylation of the ζ-chain, respectively.

Original languageEnglish
Pages (from-to)1010-1014
Number of pages5
JournalJournal of Immunology
Volume144
Issue number3
Publication statusPublished - Feb 1 1990

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CD2 Antigens
T-Cell Antigen Receptor
Tyrosine
Phosphorylation
T-Lymphocytes
Phorbol 12,13-Dibutyrate
Protein-Tyrosine Kinases
Protein Kinase C
Polyacrylamide Gel Electrophoresis
Peptides
Antibodies

ASJC Scopus subject areas

  • Immunology

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Activation of human T lymphocytes via the CD2 antigen results in tyrosine phosphorylation of T cell antigen receptor ζ-chains. / Monostori, E.; Desai, Dev; Brown, Marion H.; Cantrell, Doreen A.; Crumpton, Michael J.

In: Journal of Immunology, Vol. 144, No. 3, 01.02.1990, p. 1010-1014.

Research output: Contribution to journalArticle

Monostori, E. ; Desai, Dev ; Brown, Marion H. ; Cantrell, Doreen A. ; Crumpton, Michael J. / Activation of human T lymphocytes via the CD2 antigen results in tyrosine phosphorylation of T cell antigen receptor ζ-chains. In: Journal of Immunology. 1990 ; Vol. 144, No. 3. pp. 1010-1014.
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