An established cell line transformed by herpes simplex virus type 2 and derived from hamster tumors contained herpesvirus specific intraeellular and surface antigens in about 2-5 per cent of cells but did not produce infectious virus. Arginine deprivation resulted in induction of virus specific intracellular and surface antigens in 70-75 and about 40 per cent of cells, respectively, while no infectious virus was induced. After treatment of the cultures with IUDR 70-75 per cent of the cells showed virus specific intracellular fluorescence and 45-50 per cent exhibited cell membrane fluorescence and infectious virus could be detected. The induction by IUDR could be inhibited by simultaneous treatment of cultures with cytosine arabinoside (ara-C).
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology