Activation of embryogenic cell division in leaf protoplast-derived alfalfa cells: The role of auxin and stress

A. Fehér, T. Pasternak, D. Dudits

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Leaf protoplast-derived cells of the embryogenic alfalfa genotype (Medicago sativa ssp. varia A2) follow different fate if cultured in the presence of 0.25, 1 or 10 μM 2,4-dichlorophenoxyacetic acid (2,4-D). Cells grown in the presence of the highest auxin (2,4-D) concentration become embryogenic and can develop into somatic embryos if subcultured into fresh medium with the lower 2,4-D level. Cells cultured at the lower auxin concentrations from the beginning develop into elongated differentiated cells. In order to reveal physiological changes that characterize the reactivation of cell division in resting cells as well as the transition of somatic plant cells to an embryogenic state, morphological, cell division, intracellular pH and stress-related parameters have been determined during the first five days of parallel cultures at the above 2,4-D concentrations in combination with stress treatments.

Original languageEnglish
Pages (from-to)13-14
Number of pages2
JournalActa Biologica Szegediensis
Volume46
Issue number3-4
Publication statusPublished - 2002

Fingerprint

2,4-Dichlorophenoxyacetic Acid
Indoleacetic Acids
Medicago sativa
Protoplasts
Cell Division
protoplasts
2,4-D
cell division
alfalfa
auxins
Chemical activation
Cells
leaves
cells
Plant Cells
somatic embryos
varespladib methyl
cultured cells
Cultured Cells
Embryonic Structures

Keywords

  • 2,4-dichlorophenoxyacetic acid
  • Auxin
  • Cell cycle reactivation
  • pH gradients
  • Somatic embryogenesis
  • Stress

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Cell Biology
  • Applied Microbiology and Biotechnology
  • Neuroscience(all)

Cite this

Activation of embryogenic cell division in leaf protoplast-derived alfalfa cells : The role of auxin and stress. / Fehér, A.; Pasternak, T.; Dudits, D.

In: Acta Biologica Szegediensis, Vol. 46, No. 3-4, 2002, p. 13-14.

Research output: Contribution to journalArticle

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