Activation of deoxycytidine kinase by various nucleoside analogues

T. Spasokoukotskaja, M. Sasvári, Lehel Hullán, Freidoun Albertioni, Staffan Eriksson, M. Staub

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The effect of different nucleoside analogues on deoxycytidine kinase (dCK) and thymidine kinase (TK) was compared in normal human lymphocytes and various leukemic cell lines. G-phase enriched tonsilar lymphocyte subpopulation treated by CdA showed more profound stimulation of dCK activity than S-phase cells. No substantial changes in TK activity were detected. CdA treatment increased the activity of dCK 4-fold in peripheral blood mononuclear cells (PBMC) and 2-fold in promyelocytic cell line HL60, too. However, no significant stimulation was detected either in CCRF-CEM or in K562 cell lines. 2-Cl-2'deoxy-2'F-adenine arabinoside (CAFdA), 2F-adenine arabinoside (F-araA) and cytosine arabinoside (AraC) had the same effect as CdA, although higher concentrations were needed for maximal activation. In contrast, treatment by dCyd caused slight inhibition of dCK. The possibility of interference of nucleoside analogues with the mechanisms of posttranslational modification of dCK was proposed.

Original languageEnglish
Pages (from-to)641-645
Number of pages5
JournalAdvances in Experimental Medicine and Biology
Volume431
Publication statusPublished - 1998

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Deoxycytidine Kinase
Nucleosides
Chemical activation
Vidarabine
Lymphocytes
Thymidine Kinase
Cells
Cell Line
K562 Cells
Cytarabine
Lymphocyte Subsets
Post Translational Protein Processing
S Phase
Blood Cells
Blood

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Activation of deoxycytidine kinase by various nucleoside analogues. / Spasokoukotskaja, T.; Sasvári, M.; Hullán, Lehel; Albertioni, Freidoun; Eriksson, Staffan; Staub, M.

In: Advances in Experimental Medicine and Biology, Vol. 431, 1998, p. 641-645.

Research output: Contribution to journalArticle

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AU - Sasvári, M.

AU - Hullán, Lehel

AU - Albertioni, Freidoun

AU - Eriksson, Staffan

AU - Staub, M.

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N2 - The effect of different nucleoside analogues on deoxycytidine kinase (dCK) and thymidine kinase (TK) was compared in normal human lymphocytes and various leukemic cell lines. G-phase enriched tonsilar lymphocyte subpopulation treated by CdA showed more profound stimulation of dCK activity than S-phase cells. No substantial changes in TK activity were detected. CdA treatment increased the activity of dCK 4-fold in peripheral blood mononuclear cells (PBMC) and 2-fold in promyelocytic cell line HL60, too. However, no significant stimulation was detected either in CCRF-CEM or in K562 cell lines. 2-Cl-2'deoxy-2'F-adenine arabinoside (CAFdA), 2F-adenine arabinoside (F-araA) and cytosine arabinoside (AraC) had the same effect as CdA, although higher concentrations were needed for maximal activation. In contrast, treatment by dCyd caused slight inhibition of dCK. The possibility of interference of nucleoside analogues with the mechanisms of posttranslational modification of dCK was proposed.

AB - The effect of different nucleoside analogues on deoxycytidine kinase (dCK) and thymidine kinase (TK) was compared in normal human lymphocytes and various leukemic cell lines. G-phase enriched tonsilar lymphocyte subpopulation treated by CdA showed more profound stimulation of dCK activity than S-phase cells. No substantial changes in TK activity were detected. CdA treatment increased the activity of dCK 4-fold in peripheral blood mononuclear cells (PBMC) and 2-fold in promyelocytic cell line HL60, too. However, no significant stimulation was detected either in CCRF-CEM or in K562 cell lines. 2-Cl-2'deoxy-2'F-adenine arabinoside (CAFdA), 2F-adenine arabinoside (F-araA) and cytosine arabinoside (AraC) had the same effect as CdA, although higher concentrations were needed for maximal activation. In contrast, treatment by dCyd caused slight inhibition of dCK. The possibility of interference of nucleoside analogues with the mechanisms of posttranslational modification of dCK was proposed.

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