Activation of capsaicin-sensitive primary sensory neurones induces anandamide production and release

J. Ahluwalia, Mohammed Yaqoob, Laszlo Urban, Stuart Bevan, I. Nagy

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

The inhibitory cannabinoid 1 receptor and the excitatory vanilloid receptor 1, both of which are responsive to the endogenous ligand anandamide, are co-expressed on a subpopulation of primary sensory neurones. We report that activation of the cannabinoid 1 receptor/vanilloid receptor 1-co-expressing primary sensory neurones induces the production and release of anandamide. Application of capsaicin (3 nM-1 μM) to cultured primary sensory neurones evoked calcitonin gene-related peptide release, which was significantly increased by the selective cannabinoid 1 receptor antagonist, SR141716A (200 nM). Mass spectrometric analyses of the extracellular solution revealed that exposure to capsaicin (10 nM or 100 nM) enhanced the anandamide concentration of the medium from less then 0.05 pmol/μL to more then 2 pmol/μL. Depolarization of the neurones with 50 mM KCl also enhanced the anandamide content of the buffer. Both the capsaicin- and KCl-induced anandamide release depended on extracellular Ca2+. Prolonged treatment of the cultures with capsaicin (10 μM) reduced both the capsaicin- and KCl-induced anandamide release. These findings indicate that activation of capsaicin-sensitive primary sensory neurones evokes anandamide production and release, and that anandamide might be a key endogenous regulator of the excitability of these neurones.

Original languageEnglish
Pages (from-to)585-591
Number of pages7
JournalJournal of Neurochemistry
Volume84
Issue number3
DOIs
Publication statusPublished - Feb 2003

Fingerprint

Capsaicin
Sensory Receptor Cells
Neurons
Chemical activation
Cannabinoids
Cannabinoid Receptors
rimonabant
Cannabinoid Receptor Antagonists
anandamide
Calcitonin Gene-Related Peptide
Depolarization
Buffers
Ligands

Keywords

  • Cannabinoid 1 receptor
  • Dorsal root ganglion
  • Heat hyperalgesia
  • Inflammation
  • Nociception
  • Vanilloid receptor 1

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Activation of capsaicin-sensitive primary sensory neurones induces anandamide production and release. / Ahluwalia, J.; Yaqoob, Mohammed; Urban, Laszlo; Bevan, Stuart; Nagy, I.

In: Journal of Neurochemistry, Vol. 84, No. 3, 02.2003, p. 585-591.

Research output: Contribution to journalArticle

Ahluwalia, J. ; Yaqoob, Mohammed ; Urban, Laszlo ; Bevan, Stuart ; Nagy, I. / Activation of capsaicin-sensitive primary sensory neurones induces anandamide production and release. In: Journal of Neurochemistry. 2003 ; Vol. 84, No. 3. pp. 585-591.
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