Activation and desensitisation of acetylcholine release by zinc at Torpedo nerve terminals

Yves Dunant, Françoise Loctin, Jean Paul Vallée, A. Párducz, Bernard Lesbats, Maurice Israël

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Treatment with 100 or 250 μM ZnCl2 irreversibly blocked neurotransmission in the Torpedo electric organ by inhibiting acetylcholine (ACh) release. In Zn2+-treated tissue, release failure did not result from impairment of Ca2+ entry since stimulation still provoked an accumulation of Ca2+. Also pretreatment of isolated synaptosomes with Zn2+ inhibited to the same extent the release elicited by KCl-evoked depolarisation and the release elicited by using the Ca2+ ionophore A23187. On the other hand, after application of A23187, Zn2+ by itself efficiently triggered ACh release from synapto-somes. This dual effect of Zn2+ was also observed to occur in proteoliposomes equipped with mediatophore (a protein of the presynaptic membrane characterised by its capability to support Ca2+-dependent transmitter release). Hence, Zn2+ mimicked two fundamental actions of Ca2+ on nerve terminals, which are: (1) the immediate activation of release, and (2) a more slowly developing desensitisation of release. Zn2+ was more powerful than Ca2+ for both actions. It is concluded that the dual action of Zn2+ on the mediatophore protein accounts at least in part for its complex effects on neurotransmission.

Original languageEnglish
Pages (from-to)853-858
Number of pages6
JournalPflügers Archiv für die Gesamte Physiologie des Menschen und der Tiere
Volume432
Issue number5
DOIs
Publication statusPublished - Sep 1996

Fingerprint

Torpedo
Calcimycin
Synaptic Transmission
Acetylcholine
Zinc
Chemical activation
Electric Organ
Synaptosomes
Ionophores
Depolarization
Transmitters
Membrane Proteins
Proteins
Tissue
Membranes
mediatophore
proteoliposomes

Keywords

  • Acetylcholine release
  • Calcium
  • Mediatophore
  • Synaptic transmission
  • Synaptosomes
  • Torpedo electric organ
  • Zinc

ASJC Scopus subject areas

  • Physiology (medical)
  • Physiology
  • Clinical Biochemistry

Cite this

Activation and desensitisation of acetylcholine release by zinc at Torpedo nerve terminals. / Dunant, Yves; Loctin, Françoise; Vallée, Jean Paul; Párducz, A.; Lesbats, Bernard; Israël, Maurice.

In: Pflügers Archiv für die Gesamte Physiologie des Menschen und der Tiere, Vol. 432, No. 5, 09.1996, p. 853-858.

Research output: Contribution to journalArticle

Dunant, Yves ; Loctin, Françoise ; Vallée, Jean Paul ; Párducz, A. ; Lesbats, Bernard ; Israël, Maurice. / Activation and desensitisation of acetylcholine release by zinc at Torpedo nerve terminals. In: Pflügers Archiv für die Gesamte Physiologie des Menschen und der Tiere. 1996 ; Vol. 432, No. 5. pp. 853-858.
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