Activated platelet-derived microparticle numbers are elevated in patients with severe fungal (Candida albicans) sepsis

Gábor Woth, Margit Tokés-Füzesi, T. Magyarlaki, G. Kovács, István Vermes, Diana Mühl

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Background: The treatment of severe sepsis highly depends on the identification of bacteria or fungi from blood and/or other body materials. Although widely available blood culturing and risk assessment scores are not completely reliable, current guidelines do not recommend the wide empirical use of antifungal medications based on questionable benefit or possible side-effects. We aimed totest whether platelet-derived microparticle (MP) measurements can improve the early detection of the infective agent behind sepsis. Methods: Thirty-three consecutive severe septic patients from our university intensive care unit were included in our prospective study. MP number and surface antigen characteristics were followed by flow cytometry on days 1 (admission), 3 and 5. For microbiological identification, various specimens were collected on admission and in case of overall status deterioration. Results: On admission,septic patients showed elevated annexin V and constitutive platelet marker (CD41)-positive MP numbers compared with volunteers. Mixed fungal septic patients showed significantly elevated annexin V and CD41-positive particle numbers on day 1 (P

Original languageEnglish
Pages (from-to)554-560
Number of pages7
JournalAnnals of Clinical Biochemistry
Volume49
Issue number6
DOIs
Publication statusPublished - 2012

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Candida
Annexin A5
Platelets
Candida albicans
Sepsis
Blood
Blood Platelets
Intensive care units
Flow cytometry
Patient Admission
Surface Antigens
Fungi
Risk assessment
Intensive Care Units
Deterioration
Volunteers
Bacteria
Flow Cytometry
Prospective Studies
Guidelines

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Activated platelet-derived microparticle numbers are elevated in patients with severe fungal (Candida albicans) sepsis. / Woth, Gábor; Tokés-Füzesi, Margit; Magyarlaki, T.; Kovács, G.; Vermes, István; Mühl, Diana.

In: Annals of Clinical Biochemistry, Vol. 49, No. 6, 2012, p. 554-560.

Research output: Contribution to journalArticle

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AU - Woth, Gábor

AU - Tokés-Füzesi, Margit

AU - Magyarlaki, T.

AU - Kovács, G.

AU - Vermes, István

AU - Mühl, Diana

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AB - Background: The treatment of severe sepsis highly depends on the identification of bacteria or fungi from blood and/or other body materials. Although widely available blood culturing and risk assessment scores are not completely reliable, current guidelines do not recommend the wide empirical use of antifungal medications based on questionable benefit or possible side-effects. We aimed totest whether platelet-derived microparticle (MP) measurements can improve the early detection of the infective agent behind sepsis. Methods: Thirty-three consecutive severe septic patients from our university intensive care unit were included in our prospective study. MP number and surface antigen characteristics were followed by flow cytometry on days 1 (admission), 3 and 5. For microbiological identification, various specimens were collected on admission and in case of overall status deterioration. Results: On admission,septic patients showed elevated annexin V and constitutive platelet marker (CD41)-positive MP numbers compared with volunteers. Mixed fungal septic patients showed significantly elevated annexin V and CD41-positive particle numbers on day 1 (P

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