Action spectra for photoinduced inactivation of bacteriophage T7 sensitized by 8-methoxypsoralen and angelicin

G. Rontó, Andrea Fekete, Sándor Gáspár, K. Módos

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The action spectrum (240 - 300 nm) for photoinactivation of unsensitized phage T7 and the action spectra (310 - 380 nm) for photoinactivation of phage T7 sensitized with 8-methoxypsoralen (8-MOP) and angelicin were measured by an automated method. For unsensitized phage T7 the action spectrum is in good agreement with the absorption spectrum. For sensitization with angelicin the action spectrum is similar to the absorption spectrum, but for sensitization with 8-MOP the spectra are different. The agreement between the T7 absorption and action spectra in the far-UV region is due to photodamage of DNA, leading to phage inactivation. The similarity in the action and absorption spectra in the near-UV region for sensitization with angelicin seems to be in accordance with the monofunctional photobinding of angelicin to DNA. The action spectrum for sensitization with 8-MOP has a maximum at about 320 nm and this suggests that, in addition to the monoadducts, the biadducts play a role in the inactivation of phage T7. Taking the number of bound furocoumarin molecules into consideration, the quantum efficiencies were estimated. Furocoumarin increases the quantum efficiency in the near-UV region and the values are similar to those obtained in far-UV light without psoralens.

Original languageEnglish
Pages (from-to)497-507
Number of pages11
JournalJournal of Photochemistry and Photobiology, B: Biology
Volume3
Issue number4
DOIs
Publication statusPublished - 1989

Fingerprint

methoxsalen
Bacteriophage T7
Methoxsalen
bacteriophages
Bacteriophages
deactivation
inactivation
psoralens
Absorption spectra
Quantum efficiency
absorption spectra
DNA
quantum efficiency
deoxyribonucleic acid
Ultraviolet radiation
ultraviolet radiation
Ultraviolet Rays
angelicin
Action Spectrum
Molecules

Keywords

  • 8-MOP
  • action spectra
  • angelicin
  • photoinactivation
  • Photosensitization
  • quantum efficiency.
  • T7 phage

ASJC Scopus subject areas

  • Plant Science
  • Bioengineering
  • Physical and Theoretical Chemistry

Cite this

Action spectra for photoinduced inactivation of bacteriophage T7 sensitized by 8-methoxypsoralen and angelicin. / Rontó, G.; Fekete, Andrea; Gáspár, Sándor; Módos, K.

In: Journal of Photochemistry and Photobiology, B: Biology, Vol. 3, No. 4, 1989, p. 497-507.

Research output: Contribution to journalArticle

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