Action of chloroquine on nitric oxide production and parasite killing by macrophages

A. Hrabák, Hassan Sefrioui, Vincent Vercruysse, Ágnes Temesi, Tamás Bajor, Bernard Vray

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Chloroquine is known to inhibit several functions of macrophages, but its effect on the nitric oxide (NO)-dependent parasite killing capacity of macrophages has not been documented. NO synthesis by interferon-γ-induced mouse and casein-elicited rat macrophages was significantly and irreversibly inhibited by chloroquine. The activity of the inducible NO synthase was not directly altered, but previous incubation of macrophages with chloroquine decreased it. Chloroquine did not alter arginase activity or arginine uptake. NADPH diaphorase activity, an indicator of NO synthase was impaired. Western blotting showed that inducible NO synthase synthesis was blocked by chloroquine. The blocking of NO formation by chloroquine resulted in increased infection of mouse peritoneal macrophages by Trypanosoma cruzi (T. cruzi). This suggests that chloroquine decreases NO formation by macrophages by inhibiting the induction of NO synthase. The findings are further evidence that NO is involved in the anti-parasitic response of macrophages. Copyright (C) 1998 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)83-90
Number of pages8
JournalEuropean Journal of Pharmacology
Volume354
Issue number1
DOIs
Publication statusPublished - Jul 31 1998

Fingerprint

Chloroquine
Nitric Oxide
Parasites
Macrophages
Nitric Oxide Synthase Type II
Nitric Oxide Synthase
NADPH Dehydrogenase
Arginase
Trypanosoma cruzi
Peritoneal Macrophages
Caseins
Interferons
Arginine
Western Blotting
Infection

Keywords

  • Chloroquine
  • Inhibition of NO production
  • Macrophage
  • Nitric oxide (NO)
  • Trypanosoma cruzi infection

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Pharmacology

Cite this

Action of chloroquine on nitric oxide production and parasite killing by macrophages. / Hrabák, A.; Sefrioui, Hassan; Vercruysse, Vincent; Temesi, Ágnes; Bajor, Tamás; Vray, Bernard.

In: European Journal of Pharmacology, Vol. 354, No. 1, 31.07.1998, p. 83-90.

Research output: Contribution to journalArticle

Hrabák, A. ; Sefrioui, Hassan ; Vercruysse, Vincent ; Temesi, Ágnes ; Bajor, Tamás ; Vray, Bernard. / Action of chloroquine on nitric oxide production and parasite killing by macrophages. In: European Journal of Pharmacology. 1998 ; Vol. 354, No. 1. pp. 83-90.
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