Action of a homogeneous hydrogenation catalyst on living Tetrahymena mimbres cells

Yunbae Pak, Ferenc Joó, L. Vígh, A. Kathó, Guy A. Thompson

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Various conditions were tested in an attempt to hydrogenate the unsaturated fatty acids of living Tetrahymena mimbres with the homogeneous catalyst palladium di-(sodium alizarine monosulfonate) without causing serious damage to the cells. Using a low (20 μg/ml) catalyst concentration in the external medium, hydrogenation of > 20% of surface membrane lipid double bonds were obtained, but hydrogenation of intracellular mmebranes was minimal. When exposed to H2, cells preincubated with inactive catalyst for several hours and visibly loaded with the catalyst lost viability as soon as hydrogenation exceeded trace levels. Material secreted by Tetrahymena into their medium effectively inhibited hydrogenation of added oleic acid, normally a good substrate. Mucus secreteed by the cells, soluble proteins isolated from cell homogenates, bovine serum albumin, and cysteine were also inhibitory, but the inhibition could be overcome by employing higher catalyst concentrations. Although some enzymatic retroconversion of saturated lipids back to unsturated lipids appeared to take place, the scale of the conversion was small, and further experimentation will be required to understand the mechanism involved. The selective hydrogenation of surface membranes achieved by these methods may be especially useful to those interested in fluidity effects on plasma membrane properties.

Original languageEnglish
Pages (from-to)230-238
Number of pages9
JournalBBA - Biomembranes
Volume1023
Issue number2
DOIs
Publication statusPublished - Apr 13 1990

Fingerprint

Tetrahymena
Hydrogenation
Catalysts
Lipids
Fluidity
Mucus
Cell membranes
Oleic Acid
Membrane Lipids
Bovine Serum Albumin
Unsaturated Fatty Acids
Cysteine
Cell Membrane
Membranes
Substrates
Proteins

Keywords

  • (Tetrahymena mimbres)
  • Hydrogation catalyst
  • Lipid hydrogenation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology

Cite this

Action of a homogeneous hydrogenation catalyst on living Tetrahymena mimbres cells. / Pak, Yunbae; Joó, Ferenc; Vígh, L.; Kathó, A.; Thompson, Guy A.

In: BBA - Biomembranes, Vol. 1023, No. 2, 13.04.1990, p. 230-238.

Research output: Contribution to journalArticle

Pak, Yunbae ; Joó, Ferenc ; Vígh, L. ; Kathó, A. ; Thompson, Guy A. / Action of a homogeneous hydrogenation catalyst on living Tetrahymena mimbres cells. In: BBA - Biomembranes. 1990 ; Vol. 1023, No. 2. pp. 230-238.
@article{c583b61b05544f838eb5d68812bdfc01,
title = "Action of a homogeneous hydrogenation catalyst on living Tetrahymena mimbres cells",
abstract = "Various conditions were tested in an attempt to hydrogenate the unsaturated fatty acids of living Tetrahymena mimbres with the homogeneous catalyst palladium di-(sodium alizarine monosulfonate) without causing serious damage to the cells. Using a low (20 μg/ml) catalyst concentration in the external medium, hydrogenation of > 20{\%} of surface membrane lipid double bonds were obtained, but hydrogenation of intracellular mmebranes was minimal. When exposed to H2, cells preincubated with inactive catalyst for several hours and visibly loaded with the catalyst lost viability as soon as hydrogenation exceeded trace levels. Material secreted by Tetrahymena into their medium effectively inhibited hydrogenation of added oleic acid, normally a good substrate. Mucus secreteed by the cells, soluble proteins isolated from cell homogenates, bovine serum albumin, and cysteine were also inhibitory, but the inhibition could be overcome by employing higher catalyst concentrations. Although some enzymatic retroconversion of saturated lipids back to unsturated lipids appeared to take place, the scale of the conversion was small, and further experimentation will be required to understand the mechanism involved. The selective hydrogenation of surface membranes achieved by these methods may be especially useful to those interested in fluidity effects on plasma membrane properties.",
keywords = "(Tetrahymena mimbres), Hydrogation catalyst, Lipid hydrogenation",
author = "Yunbae Pak and Ferenc Jo{\'o} and L. V{\'i}gh and A. Kath{\'o} and Thompson, {Guy A.}",
year = "1990",
month = "4",
day = "13",
doi = "10.1016/0005-2736(90)90418-N",
language = "English",
volume = "1023",
pages = "230--238",
journal = "Biochimica et Biophysica Acta - Biomembranes",
issn = "0005-2736",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - Action of a homogeneous hydrogenation catalyst on living Tetrahymena mimbres cells

AU - Pak, Yunbae

AU - Joó, Ferenc

AU - Vígh, L.

AU - Kathó, A.

AU - Thompson, Guy A.

PY - 1990/4/13

Y1 - 1990/4/13

N2 - Various conditions were tested in an attempt to hydrogenate the unsaturated fatty acids of living Tetrahymena mimbres with the homogeneous catalyst palladium di-(sodium alizarine monosulfonate) without causing serious damage to the cells. Using a low (20 μg/ml) catalyst concentration in the external medium, hydrogenation of > 20% of surface membrane lipid double bonds were obtained, but hydrogenation of intracellular mmebranes was minimal. When exposed to H2, cells preincubated with inactive catalyst for several hours and visibly loaded with the catalyst lost viability as soon as hydrogenation exceeded trace levels. Material secreted by Tetrahymena into their medium effectively inhibited hydrogenation of added oleic acid, normally a good substrate. Mucus secreteed by the cells, soluble proteins isolated from cell homogenates, bovine serum albumin, and cysteine were also inhibitory, but the inhibition could be overcome by employing higher catalyst concentrations. Although some enzymatic retroconversion of saturated lipids back to unsturated lipids appeared to take place, the scale of the conversion was small, and further experimentation will be required to understand the mechanism involved. The selective hydrogenation of surface membranes achieved by these methods may be especially useful to those interested in fluidity effects on plasma membrane properties.

AB - Various conditions were tested in an attempt to hydrogenate the unsaturated fatty acids of living Tetrahymena mimbres with the homogeneous catalyst palladium di-(sodium alizarine monosulfonate) without causing serious damage to the cells. Using a low (20 μg/ml) catalyst concentration in the external medium, hydrogenation of > 20% of surface membrane lipid double bonds were obtained, but hydrogenation of intracellular mmebranes was minimal. When exposed to H2, cells preincubated with inactive catalyst for several hours and visibly loaded with the catalyst lost viability as soon as hydrogenation exceeded trace levels. Material secreted by Tetrahymena into their medium effectively inhibited hydrogenation of added oleic acid, normally a good substrate. Mucus secreteed by the cells, soluble proteins isolated from cell homogenates, bovine serum albumin, and cysteine were also inhibitory, but the inhibition could be overcome by employing higher catalyst concentrations. Although some enzymatic retroconversion of saturated lipids back to unsturated lipids appeared to take place, the scale of the conversion was small, and further experimentation will be required to understand the mechanism involved. The selective hydrogenation of surface membranes achieved by these methods may be especially useful to those interested in fluidity effects on plasma membrane properties.

KW - (Tetrahymena mimbres)

KW - Hydrogation catalyst

KW - Lipid hydrogenation

UR - http://www.scopus.com/inward/record.url?scp=0025355450&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025355450&partnerID=8YFLogxK

U2 - 10.1016/0005-2736(90)90418-N

DO - 10.1016/0005-2736(90)90418-N

M3 - Article

C2 - 2328248

AN - SCOPUS:0025355450

VL - 1023

SP - 230

EP - 238

JO - Biochimica et Biophysica Acta - Biomembranes

JF - Biochimica et Biophysica Acta - Biomembranes

SN - 0005-2736

IS - 2

ER -