Actin-filament motion in the in vitro motility assay has a periodic component

Evert L. DeBeer, Annemiek M A T A Sontrop, M. Kellermayer, Csaba Galambos, Gerald H. Pollack

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The interaction between actin and myosin can be studied in the in vitro motility assay, where fluorescently labelled actin filaments are observed to move over a lawn of myosin heads. To examine details of this movement, we measured systematically the velocities of the front end, rear end, and centroid of the actin filament as the filament translated over the assay surface. We found that these velocities exhibited an unexpectedly periodic component, alternating regularly between high and low values, superimposed on the steady velocity component. The period of the oscillatory component was approximately 380 ms. When translation was stopped by an increase in osmolarity, the filaments wiggled with a periodicity similar to the translating filament, implying that wiggling and translation may be related. Rigor filaments showed no periodicity. From the frequency content of the auto- and cross-correlation functions derived from the velocities of the front end, rear end, and centroid of the actin filament, we infer a deterministic, possibly wave-like process travelling along the actin filament. Potential molecular mechanisms underlying this phenomenon are considered.

Original languageEnglish
Pages (from-to)341-350
Number of pages10
JournalCell Motility and the Cytoskeleton
Volume38
Issue number4
DOIs
Publication statusPublished - 1997

Fingerprint

Actin Cytoskeleton
Periodicity
Myosins
Osmolar Concentration
Actins
In Vitro Techniques

Keywords

  • Autocorrelation
  • Cross-correlation
  • Reputation
  • Velocity oscillation

ASJC Scopus subject areas

  • Cell Biology

Cite this

Actin-filament motion in the in vitro motility assay has a periodic component. / DeBeer, Evert L.; Sontrop, Annemiek M A T A; Kellermayer, M.; Galambos, Csaba; Pollack, Gerald H.

In: Cell Motility and the Cytoskeleton, Vol. 38, No. 4, 1997, p. 341-350.

Research output: Contribution to journalArticle

DeBeer, Evert L. ; Sontrop, Annemiek M A T A ; Kellermayer, M. ; Galambos, Csaba ; Pollack, Gerald H. / Actin-filament motion in the in vitro motility assay has a periodic component. In: Cell Motility and the Cytoskeleton. 1997 ; Vol. 38, No. 4. pp. 341-350.
@article{d63264b31fa94fcdbb998cd00cd0681f,
title = "Actin-filament motion in the in vitro motility assay has a periodic component",
abstract = "The interaction between actin and myosin can be studied in the in vitro motility assay, where fluorescently labelled actin filaments are observed to move over a lawn of myosin heads. To examine details of this movement, we measured systematically the velocities of the front end, rear end, and centroid of the actin filament as the filament translated over the assay surface. We found that these velocities exhibited an unexpectedly periodic component, alternating regularly between high and low values, superimposed on the steady velocity component. The period of the oscillatory component was approximately 380 ms. When translation was stopped by an increase in osmolarity, the filaments wiggled with a periodicity similar to the translating filament, implying that wiggling and translation may be related. Rigor filaments showed no periodicity. From the frequency content of the auto- and cross-correlation functions derived from the velocities of the front end, rear end, and centroid of the actin filament, we infer a deterministic, possibly wave-like process travelling along the actin filament. Potential molecular mechanisms underlying this phenomenon are considered.",
keywords = "Autocorrelation, Cross-correlation, Reputation, Velocity oscillation",
author = "DeBeer, {Evert L.} and Sontrop, {Annemiek M A T A} and M. Kellermayer and Csaba Galambos and Pollack, {Gerald H.}",
year = "1997",
doi = "10.1002/(SICI)1097-0169(1997)38:4<341::AID-CM4>3.0.CO;2-6",
language = "English",
volume = "38",
pages = "341--350",
journal = "Cytoskeleton",
issn = "1949-3584",
publisher = "Wiley-Liss Inc.",
number = "4",

}

TY - JOUR

T1 - Actin-filament motion in the in vitro motility assay has a periodic component

AU - DeBeer, Evert L.

AU - Sontrop, Annemiek M A T A

AU - Kellermayer, M.

AU - Galambos, Csaba

AU - Pollack, Gerald H.

PY - 1997

Y1 - 1997

N2 - The interaction between actin and myosin can be studied in the in vitro motility assay, where fluorescently labelled actin filaments are observed to move over a lawn of myosin heads. To examine details of this movement, we measured systematically the velocities of the front end, rear end, and centroid of the actin filament as the filament translated over the assay surface. We found that these velocities exhibited an unexpectedly periodic component, alternating regularly between high and low values, superimposed on the steady velocity component. The period of the oscillatory component was approximately 380 ms. When translation was stopped by an increase in osmolarity, the filaments wiggled with a periodicity similar to the translating filament, implying that wiggling and translation may be related. Rigor filaments showed no periodicity. From the frequency content of the auto- and cross-correlation functions derived from the velocities of the front end, rear end, and centroid of the actin filament, we infer a deterministic, possibly wave-like process travelling along the actin filament. Potential molecular mechanisms underlying this phenomenon are considered.

AB - The interaction between actin and myosin can be studied in the in vitro motility assay, where fluorescently labelled actin filaments are observed to move over a lawn of myosin heads. To examine details of this movement, we measured systematically the velocities of the front end, rear end, and centroid of the actin filament as the filament translated over the assay surface. We found that these velocities exhibited an unexpectedly periodic component, alternating regularly between high and low values, superimposed on the steady velocity component. The period of the oscillatory component was approximately 380 ms. When translation was stopped by an increase in osmolarity, the filaments wiggled with a periodicity similar to the translating filament, implying that wiggling and translation may be related. Rigor filaments showed no periodicity. From the frequency content of the auto- and cross-correlation functions derived from the velocities of the front end, rear end, and centroid of the actin filament, we infer a deterministic, possibly wave-like process travelling along the actin filament. Potential molecular mechanisms underlying this phenomenon are considered.

KW - Autocorrelation

KW - Cross-correlation

KW - Reputation

KW - Velocity oscillation

UR - http://www.scopus.com/inward/record.url?scp=0030779175&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030779175&partnerID=8YFLogxK

U2 - 10.1002/(SICI)1097-0169(1997)38:4<341::AID-CM4>3.0.CO;2-6

DO - 10.1002/(SICI)1097-0169(1997)38:4<341::AID-CM4>3.0.CO;2-6

M3 - Article

VL - 38

SP - 341

EP - 350

JO - Cytoskeleton

JF - Cytoskeleton

SN - 1949-3584

IS - 4

ER -