ACTH radioimmunocytochemistry (RICH) on rat anterior pituitary cells

Gy Rappay, M. Kárteszi, G. Makara

Research output: Contribution to journalArticle

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Abstract

Radioimmunocytochemistry (RICH) was applied to detect corticotrophs in adult rat pituitaries and 8-day-old anterior pituitary monolayers by incubating sections and cultures with 125I-ACTH-anti ACTH immune complexes. After incubations autoradiography was made. In comparison, "conventional" immunostaining was carried out on adjacent sections and parallel cultures. It has been esteblished that RICH is suitable for detection of corticotrophs.

Original languageEnglish
Pages (from-to)207-213
Number of pages7
JournalHistochemistry
Volume59
Issue number3
DOIs
Publication statusPublished - Sep 1979

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Corticotrophs
Adrenocorticotropic Hormone
Antigen-Antibody Complex
Autoradiography

ASJC Scopus subject areas

  • Anatomy

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ACTH radioimmunocytochemistry (RICH) on rat anterior pituitary cells. / Rappay, Gy; Kárteszi, M.; Makara, G.

In: Histochemistry, Vol. 59, No. 3, 09.1979, p. 207-213.

Research output: Contribution to journalArticle

Rappay, Gy ; Kárteszi, M. ; Makara, G. / ACTH radioimmunocytochemistry (RICH) on rat anterior pituitary cells. In: Histochemistry. 1979 ; Vol. 59, No. 3. pp. 207-213.
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