Acridine orange staining of the mammalian fibroblast cell coat

J. Tímár, G. Gyapay, K. Lapis

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Acridine orange selectively binds to glycos- and galactosaminoglycan (GAG) compunds in the presence of Na+ in low concentrations. We have worked out a cytochemical method, which is suitable for the specific demonstration of surface GAG components. The method consists of a glutaraldehyde prefixation, an acridine orange block-staining for 48 h and an OsO4 postfixation for some hours. The specificity of the staining was verified with the help of fluorimetric-in vitro-measurements and enzymatic digestions, respectively.

Original languageEnglish
Pages (from-to)189-193
Number of pages5
JournalHistochemistry
Volume64
Issue number2
DOIs
Publication statusPublished - Jan 1979

Fingerprint

Glycocalyx
Acridine Orange
Fibroblasts
Staining and Labeling
Glutaral
Digestion
galactosaminoglycan
In Vitro Techniques

ASJC Scopus subject areas

  • Anatomy

Cite this

Acridine orange staining of the mammalian fibroblast cell coat. / Tímár, J.; Gyapay, G.; Lapis, K.

In: Histochemistry, Vol. 64, No. 2, 01.1979, p. 189-193.

Research output: Contribution to journalArticle

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