AcpA, a member of the GPR1/FUN34/YaaH membrane protein family, is essential for acetate permease activity in the hyphal fungus Aspergillus nidulans

Xavier Robellet, M. Flipphi, Sylvine Pégot, Andrew P. MacCabe, Christian Vélot

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

In a previous study, alcS, a gene of the Aspergillus nidulans ale cluster, was shown to encode a protein that belongs to the GPR1/FUN34/YaaH membrane protein family. BLAST screening of the A. nidulans genome data identified additional genes encoding hypothetical proteins that could belong to this family. In this study we report the functional characterization of one of them, AN5226. Its expression is induced by ethanol and ethyl acetate (two inducers of the ale genes) and is mediated by the specific transcriptional activator of genes of the acetate-utilization pathway FacB. Growth of a null mutant (ΔAN5226) is notably affected when acetate is used as sole carbon source at low concentration and in a high pH medium, i.e. when protonated acetate, the form that can enter the cell by passive diffusion, is present in low amounts. Consistently, expression of AN5226 is also induced by acetate, but only when the latter is present at low concentrations. 14C-labelled acetate uptake experiments using germinating conidia demonstrate an essential role for AN5226 in mediated acetate transport. To our knowledge this report is the first to provide evidence for the identification of an acetate transporter in filamentous fungi. We have designated AN5226 as acpA (for acetate permease A).

Original languageEnglish
Pages (from-to)485-493
Number of pages9
JournalBiochemical Journal
Volume412
Issue number3
DOIs
Publication statusPublished - Jun 15 2008

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Aspergillus nidulans
Membrane Transport Proteins
Aspergillus
Fungi
Membrane Proteins
Acetates
Genes
Gene encoding
Fungal Spores
Screening
Proteins
Ethanol
Carbon
Genome
Growth

Keywords

  • Acetate
  • Aspergillus nidulans
  • Gene expression
  • GPR1/FUN34/YaaH protein family
  • Monocarboxylate transport

ASJC Scopus subject areas

  • Biochemistry

Cite this

AcpA, a member of the GPR1/FUN34/YaaH membrane protein family, is essential for acetate permease activity in the hyphal fungus Aspergillus nidulans. / Robellet, Xavier; Flipphi, M.; Pégot, Sylvine; MacCabe, Andrew P.; Vélot, Christian.

In: Biochemical Journal, Vol. 412, No. 3, 15.06.2008, p. 485-493.

Research output: Contribution to journalArticle

Robellet, Xavier ; Flipphi, M. ; Pégot, Sylvine ; MacCabe, Andrew P. ; Vélot, Christian. / AcpA, a member of the GPR1/FUN34/YaaH membrane protein family, is essential for acetate permease activity in the hyphal fungus Aspergillus nidulans. In: Biochemical Journal. 2008 ; Vol. 412, No. 3. pp. 485-493.
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