Acetylcholine controls the gain of the voltage-to-movement converter in isolated outer hair cells

Istvan Sziklai, Peter Dallos

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Sensitivity of the electromotility of isolated guinea pig outer hair cells (OHCs) to acetylcholine (ACh) was examined. OHCs were held in a partitioning microchamber in a position so that their ciliary poles were inserted and their somatic length changes measured. Transcellular square-wave stimuli were delivered to record voltage-to-movement conversion of the cells. ACh was applied to the synaptic poles. The transfer function of electromotility intimated shifts of the membrane potential with concomitant gain changes: gain decreases when the membrane potential is shifted in hyperpolarization direction and gain increases when the membrane potential is shifted in the opposite direction due to application of ACh in 100-500/JM concentration. Clear gain increase of the electromotility in basal turn OHCs to ACh was observed, whereas inconsistent results for apical turn OHCs were found. The latter is probably due to the pharmacological dose of ACh used. This possibility is further supported by results in which ACh abolished the sensitivity of the magnitude response of electromotility to DC depolarizing bias.

Original languageEnglish
Pages (from-to)326-329
Number of pages4
JournalActa Oto-Laryngologica
Volume113
Issue number3
DOIs
Publication statusPublished - Jan 1 1993

Keywords

  • Acetylcholine
  • Electromotility
  • Isolated outer hair cell
  • Transfer function

ASJC Scopus subject areas

  • Otorhinolaryngology

Fingerprint Dive into the research topics of 'Acetylcholine controls the gain of the voltage-to-movement converter in isolated outer hair cells'. Together they form a unique fingerprint.

  • Cite this