Ace2p controls the expression of genes required for cell separation in Schizosaccharomyces pombe

Maria Luisa Alonso-Nuñez, Hanbing An, Ana Belén Martín-Cuadrado, Sapna Mehta, Claudia Petit, M. Sipiczki, Francisco Del Rey, Katheleen L. Gould, Carlos R. Vázquez De Aldana

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

Schizosaccharomyces pombe cells divide by medial fission through contraction of an actomyosin ring and deposition of a multilayered division septum that must be cleaved to release the two daughter cells. Here we describe the identification of seven genes (adg1+, adg2+, adg3 +, cfh4+, agn1+, eng1+, and mid2+) whose expression is induced by the transcription factor Ace2p. The expression of all of these genes varied during the cell cycle, maximum transcription being observed during septation. At least three of these proteins (Eng1p, Agn1p, and Cfh4p) localize to a ring-like structure that surrounds the septum region during cell separation. Deletion of the previously uncharacterized genes was not lethal to the cells, but produced defects or delays in cell separation to different extents. Electron microscopic observation of mutant cells indicated that the most severe defect is found in eng1Δ agn1Δ cells, lacking the Eng1p endo-β-1,3-glucanase and the Agn1p endo-α-glucanase. The phenotype of this mutant closely resembled that of ace2Δ mutants, forming branched chains of cells. This suggests that these two proteins are the main activities required for cell separation to be completed.

Original languageEnglish
Pages (from-to)2003-2017
Number of pages15
JournalMolecular Biology of the Cell
Volume16
Issue number4
DOIs
Publication statusPublished - Apr 2005

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Schizosaccharomyces
Cell Separation
Gene Expression
Actomyosin
Genes
Cell Cycle
Proteins
Transcription Factors
Electrons
Phenotype

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Alonso-Nuñez, M. L., An, H., Martín-Cuadrado, A. B., Mehta, S., Petit, C., Sipiczki, M., ... Vázquez De Aldana, C. R. (2005). Ace2p controls the expression of genes required for cell separation in Schizosaccharomyces pombe. Molecular Biology of the Cell, 16(4), 2003-2017. https://doi.org/10.1091/mbc.E04-06-0442

Ace2p controls the expression of genes required for cell separation in Schizosaccharomyces pombe. / Alonso-Nuñez, Maria Luisa; An, Hanbing; Martín-Cuadrado, Ana Belén; Mehta, Sapna; Petit, Claudia; Sipiczki, M.; Del Rey, Francisco; Gould, Katheleen L.; Vázquez De Aldana, Carlos R.

In: Molecular Biology of the Cell, Vol. 16, No. 4, 04.2005, p. 2003-2017.

Research output: Contribution to journalArticle

Alonso-Nuñez, ML, An, H, Martín-Cuadrado, AB, Mehta, S, Petit, C, Sipiczki, M, Del Rey, F, Gould, KL & Vázquez De Aldana, CR 2005, 'Ace2p controls the expression of genes required for cell separation in Schizosaccharomyces pombe', Molecular Biology of the Cell, vol. 16, no. 4, pp. 2003-2017. https://doi.org/10.1091/mbc.E04-06-0442
Alonso-Nuñez, Maria Luisa ; An, Hanbing ; Martín-Cuadrado, Ana Belén ; Mehta, Sapna ; Petit, Claudia ; Sipiczki, M. ; Del Rey, Francisco ; Gould, Katheleen L. ; Vázquez De Aldana, Carlos R. / Ace2p controls the expression of genes required for cell separation in Schizosaccharomyces pombe. In: Molecular Biology of the Cell. 2005 ; Vol. 16, No. 4. pp. 2003-2017.
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