Accurate measurement of long-range heteronuclear coupling constants from undistorted multiplets of an enhanced CPMG-HSQMBC experiment

K. Kövér, G. Batta, Krisztina Fehér

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Here, we present a modified CPMG-HSQMBC experiment which is capable to reduce the detrimental phase twists in the "long-range" connectivity multiplets caused by proton-proton couplings. We demonstrate that concerted CPMG pulse trains applied on both nuclei in the starting CPMG-INEPT transfer step can considerably be improved by composite π pulses that compensate for pulse imperfections and off-resonance effects. Experimental optimization of the interpulse delay within the CPMG cycle was found to be crucial in order to achieve the best possible "decoupling" of homonuclear coupling modulation.

Original languageEnglish
Pages (from-to)89-97
Number of pages9
JournalJournal of Magnetic Resonance
Volume181
Issue number1
DOIs
Publication statusPublished - Jul 2006

Fingerprint

Protons
fine structure
pulses
protons
Experiments
Modulation
decoupling
Defects
Composite materials
modulation
cycles
optimization
nuclei
composite materials
defects

Keywords

  • Carbohydrate
  • Composite pulses
  • CPMG
  • Glycopeptide
  • HSQMBC
  • Long-range heteronuclear couplings

ASJC Scopus subject areas

  • Molecular Biology
  • Physical and Theoretical Chemistry
  • Spectroscopy
  • Radiology Nuclear Medicine and imaging
  • Condensed Matter Physics

Cite this

@article{17406c864e6d4cbba455197f053a5eac,
title = "Accurate measurement of long-range heteronuclear coupling constants from undistorted multiplets of an enhanced CPMG-HSQMBC experiment",
abstract = "Here, we present a modified CPMG-HSQMBC experiment which is capable to reduce the detrimental phase twists in the {"}long-range{"} connectivity multiplets caused by proton-proton couplings. We demonstrate that concerted CPMG pulse trains applied on both nuclei in the starting CPMG-INEPT transfer step can considerably be improved by composite π pulses that compensate for pulse imperfections and off-resonance effects. Experimental optimization of the interpulse delay within the CPMG cycle was found to be crucial in order to achieve the best possible {"}decoupling{"} of homonuclear coupling modulation.",
keywords = "Carbohydrate, Composite pulses, CPMG, Glycopeptide, HSQMBC, Long-range heteronuclear couplings",
author = "K. K{\"o}v{\'e}r and G. Batta and Krisztina Feh{\'e}r",
year = "2006",
month = "7",
doi = "10.1016/j.jmr.2006.03.015",
language = "English",
volume = "181",
pages = "89--97",
journal = "Journal of Magnetic Resonance",
issn = "1090-7807",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Accurate measurement of long-range heteronuclear coupling constants from undistorted multiplets of an enhanced CPMG-HSQMBC experiment

AU - Kövér, K.

AU - Batta, G.

AU - Fehér, Krisztina

PY - 2006/7

Y1 - 2006/7

N2 - Here, we present a modified CPMG-HSQMBC experiment which is capable to reduce the detrimental phase twists in the "long-range" connectivity multiplets caused by proton-proton couplings. We demonstrate that concerted CPMG pulse trains applied on both nuclei in the starting CPMG-INEPT transfer step can considerably be improved by composite π pulses that compensate for pulse imperfections and off-resonance effects. Experimental optimization of the interpulse delay within the CPMG cycle was found to be crucial in order to achieve the best possible "decoupling" of homonuclear coupling modulation.

AB - Here, we present a modified CPMG-HSQMBC experiment which is capable to reduce the detrimental phase twists in the "long-range" connectivity multiplets caused by proton-proton couplings. We demonstrate that concerted CPMG pulse trains applied on both nuclei in the starting CPMG-INEPT transfer step can considerably be improved by composite π pulses that compensate for pulse imperfections and off-resonance effects. Experimental optimization of the interpulse delay within the CPMG cycle was found to be crucial in order to achieve the best possible "decoupling" of homonuclear coupling modulation.

KW - Carbohydrate

KW - Composite pulses

KW - CPMG

KW - Glycopeptide

KW - HSQMBC

KW - Long-range heteronuclear couplings

UR - http://www.scopus.com/inward/record.url?scp=33744973034&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33744973034&partnerID=8YFLogxK

U2 - 10.1016/j.jmr.2006.03.015

DO - 10.1016/j.jmr.2006.03.015

M3 - Article

C2 - 16621632

AN - SCOPUS:33744973034

VL - 181

SP - 89

EP - 97

JO - Journal of Magnetic Resonance

JF - Journal of Magnetic Resonance

SN - 1090-7807

IS - 1

ER -