Accurate measurement of long-range heteronuclear coupling constants from undistorted multiplets of an enhanced CPMG-HSQMBC experiment

Katalin E. Kövér, Gyula Batta, Krisztina Fehér

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Here, we present a modified CPMG-HSQMBC experiment which is capable to reduce the detrimental phase twists in the "long-range" connectivity multiplets caused by proton-proton couplings. We demonstrate that concerted CPMG pulse trains applied on both nuclei in the starting CPMG-INEPT transfer step can considerably be improved by composite π pulses that compensate for pulse imperfections and off-resonance effects. Experimental optimization of the interpulse delay within the CPMG cycle was found to be crucial in order to achieve the best possible "decoupling" of homonuclear coupling modulation.

Original languageEnglish
Pages (from-to)89-97
Number of pages9
JournalJournal of Magnetic Resonance
Volume181
Issue number1
DOIs
Publication statusPublished - Jul 1 2006

Keywords

  • CPMG
  • Carbohydrate
  • Composite pulses
  • Glycopeptide
  • HSQMBC
  • Long-range heteronuclear couplings

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Nuclear and High Energy Physics
  • Condensed Matter Physics

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