Accurate determination of small one-bond heteronuclear residual dipolar couplings by F1 coupled HSQC modified with a G- BIRD(r) module

Krisztina Fehér, Stefan Berger, K. Kövér

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

We report a G-BIRD(r) modified coupled HSQC experiment for the accurate determination of one-bond heteronuclear residual dipolar couplings. The G-BIRD(r) module has been employed to refocus the long-range coupling evolution of the heteronucleus during the t1 frequency labeling period. As a result, the crosspeaks obtained are split by only the direct one-bond coupling that can be extracted by measuring simple frequency differences between singlet maxima. Additionally the decoupling of long-range multiple bond splittings leads to considerable sensitivity enhancement. The modification also has been applied in a TROSY sequence resulting in a significant sensitivity and resolution improvement.

Original languageEnglish
Pages (from-to)340-346
Number of pages7
JournalJournal of Magnetic Resonance
Volume163
Issue number2
DOIs
Publication statusPublished - Aug 2003

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Labeling
modules
Experiments
sensitivity
decoupling
marking
augmentation

Keywords

  • Coupled HSQC
  • G-BIRD
  • One-bond heteronuclear coupling constant
  • Residual dipolar coupling constant
  • TROSY

ASJC Scopus subject areas

  • Molecular Biology
  • Physical and Theoretical Chemistry
  • Spectroscopy
  • Radiology Nuclear Medicine and imaging
  • Condensed Matter Physics

Cite this

Accurate determination of small one-bond heteronuclear residual dipolar couplings by F1 coupled HSQC modified with a G- BIRD(r) module. / Fehér, Krisztina; Berger, Stefan; Kövér, K.

In: Journal of Magnetic Resonance, Vol. 163, No. 2, 08.2003, p. 340-346.

Research output: Contribution to journalArticle

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