Accurate determination of small one-bond heteronuclear residual dipolar couplings by F1 coupled HSQC modified with a G- BIRD(r) module

Krisztina Fehér, Stefan Berger, Katalin E. Kövér

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48 Citations (Scopus)


We report a G-BIRD(r) modified coupled HSQC experiment for the accurate determination of one-bond heteronuclear residual dipolar couplings. The G-BIRD(r) module has been employed to refocus the long-range coupling evolution of the heteronucleus during the t1 frequency labeling period. As a result, the crosspeaks obtained are split by only the direct one-bond coupling that can be extracted by measuring simple frequency differences between singlet maxima. Additionally the decoupling of long-range multiple bond splittings leads to considerable sensitivity enhancement. The modification also has been applied in a TROSY sequence resulting in a significant sensitivity and resolution improvement.

Original languageEnglish
Pages (from-to)340-346
Number of pages7
JournalJournal of Magnetic Resonance
Issue number2
Publication statusPublished - Aug 1 2003



  • Coupled HSQC
  • G-BIRD
  • One-bond heteronuclear coupling constant
  • Residual dipolar coupling constant

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Nuclear and High Energy Physics
  • Condensed Matter Physics

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