Accumulation of S-d-lactoylglutathione and transient decrease of glutathione level caused by methylglyoxal load in isolated hepatocytes

M. Kalapos, Tamás Garzó, Ferenc Antoni, J. Mandl

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Abstract

Methylglyoxal is converted to d-lactic acid through a conjugation with glutathione and S-d-lactoylglutathione is an intermediate of this pathway. In isolated hepatocytes prepared from fed mice incubated without nutrients (glucose, pyruvate and amino acids) the formation and release of S-d-lactoylglutathione and also a continuous lowering of cellular glutathione were demonstrated upon addition of methylglyoxal (20 mM). Under these incubation conditions, the glutathione content of the cells decreased in the controls. On the other hand, in hepatocytes incubated in a medium supplemented with the above-mentioned compounds an accumulation of S-d-lactoylglutathione and a transient decrease of glutathione were shown after addition of methylglyoxal. Under these experimental circumstances the glutathione content of the cells was preserved. Buthionine sulfoximine - an inhibitor of glutathione synthesis - prevented the restoration of glutathione level in hepatocytes observed in the presence of methylglyoxal; emetine - an inhibitor of protein synthesis - was ineffective. It is suggested that increased methylglyoxal formation may have a role in alterations of glutathione metabolism under conditions when serum acetone in increased and methylglyoxal production from acetone is elevated.

Original languageEnglish
Pages (from-to)159-164
Number of pages6
JournalBiochimica et Biophysica Acta - Molecular Cell Research
Volume1135
Issue number2
DOIs
Publication statusPublished - Jun 10 1992

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Pyruvaldehyde
Glutathione
Hepatocytes
Acetone
Emetine
Buthionine Sulfoximine
Protein Synthesis Inhibitors
S-lactoylglutathione
Pyruvic Acid
Lactic Acid
Amino Acids
Glucose
Food

Keywords

  • Glutathione
  • Hepatocyte (Mouse)
  • Methylglyoxal
  • S-d-Lactoylglutathione

ASJC Scopus subject areas

  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

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title = "Accumulation of S-d-lactoylglutathione and transient decrease of glutathione level caused by methylglyoxal load in isolated hepatocytes",
abstract = "Methylglyoxal is converted to d-lactic acid through a conjugation with glutathione and S-d-lactoylglutathione is an intermediate of this pathway. In isolated hepatocytes prepared from fed mice incubated without nutrients (glucose, pyruvate and amino acids) the formation and release of S-d-lactoylglutathione and also a continuous lowering of cellular glutathione were demonstrated upon addition of methylglyoxal (20 mM). Under these incubation conditions, the glutathione content of the cells decreased in the controls. On the other hand, in hepatocytes incubated in a medium supplemented with the above-mentioned compounds an accumulation of S-d-lactoylglutathione and a transient decrease of glutathione were shown after addition of methylglyoxal. Under these experimental circumstances the glutathione content of the cells was preserved. Buthionine sulfoximine - an inhibitor of glutathione synthesis - prevented the restoration of glutathione level in hepatocytes observed in the presence of methylglyoxal; emetine - an inhibitor of protein synthesis - was ineffective. It is suggested that increased methylglyoxal formation may have a role in alterations of glutathione metabolism under conditions when serum acetone in increased and methylglyoxal production from acetone is elevated.",
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author = "M. Kalapos and Tam{\'a}s Garz{\'o} and Ferenc Antoni and J. Mandl",
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T1 - Accumulation of S-d-lactoylglutathione and transient decrease of glutathione level caused by methylglyoxal load in isolated hepatocytes

AU - Kalapos, M.

AU - Garzó, Tamás

AU - Antoni, Ferenc

AU - Mandl, J.

PY - 1992/6/10

Y1 - 1992/6/10

N2 - Methylglyoxal is converted to d-lactic acid through a conjugation with glutathione and S-d-lactoylglutathione is an intermediate of this pathway. In isolated hepatocytes prepared from fed mice incubated without nutrients (glucose, pyruvate and amino acids) the formation and release of S-d-lactoylglutathione and also a continuous lowering of cellular glutathione were demonstrated upon addition of methylglyoxal (20 mM). Under these incubation conditions, the glutathione content of the cells decreased in the controls. On the other hand, in hepatocytes incubated in a medium supplemented with the above-mentioned compounds an accumulation of S-d-lactoylglutathione and a transient decrease of glutathione were shown after addition of methylglyoxal. Under these experimental circumstances the glutathione content of the cells was preserved. Buthionine sulfoximine - an inhibitor of glutathione synthesis - prevented the restoration of glutathione level in hepatocytes observed in the presence of methylglyoxal; emetine - an inhibitor of protein synthesis - was ineffective. It is suggested that increased methylglyoxal formation may have a role in alterations of glutathione metabolism under conditions when serum acetone in increased and methylglyoxal production from acetone is elevated.

AB - Methylglyoxal is converted to d-lactic acid through a conjugation with glutathione and S-d-lactoylglutathione is an intermediate of this pathway. In isolated hepatocytes prepared from fed mice incubated without nutrients (glucose, pyruvate and amino acids) the formation and release of S-d-lactoylglutathione and also a continuous lowering of cellular glutathione were demonstrated upon addition of methylglyoxal (20 mM). Under these incubation conditions, the glutathione content of the cells decreased in the controls. On the other hand, in hepatocytes incubated in a medium supplemented with the above-mentioned compounds an accumulation of S-d-lactoylglutathione and a transient decrease of glutathione were shown after addition of methylglyoxal. Under these experimental circumstances the glutathione content of the cells was preserved. Buthionine sulfoximine - an inhibitor of glutathione synthesis - prevented the restoration of glutathione level in hepatocytes observed in the presence of methylglyoxal; emetine - an inhibitor of protein synthesis - was ineffective. It is suggested that increased methylglyoxal formation may have a role in alterations of glutathione metabolism under conditions when serum acetone in increased and methylglyoxal production from acetone is elevated.

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