Accumulation of renin and imidazolone in peritubular capillary endothelial cells in insulin-resistant hypertensive rats

Zoltán Wagner, Péter Degrell, Balázs Lukáts, Toshimitsu Niwa, Gergo A. Molnár, Lajos Markó, Zoltán Karádi, István Wittmann

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Background: Peritubular endothelium plays a key role in the development and progression of diabetic and nondiabetic chronic kidney disease. Renal injury in disorders of glucose metabolism may appear as early as in the stage of impaired glucose tolerance (IGT) and is accelerated by hypertension. The aim of our study was to investigate renal histology in rats with hypertension and IGT, with special emphasis on the peritubular endothelium. Methods: Hypertension and IGT (H/IGT) were provoked in adult male Wistar rats by bilateral administration of methylglyoxal into the ventromedial hypothalamus. Immunohistochemistry with anti-renin and anti-imidazolone antibodies and immunoelectron microscopy with anti-renin antibody were performed. Results: H/IGT rats showed tubulointerstitial fibrosis as well as renin and imidazolone staining in the papillary region. The patterns of immunostaining for renin and imidazolone were similar to that of endothelium. On electron microscopy, peritubular capillary endothelial cells and to a less extent, tubular epithelial cells showed renin positivity. Discussion: Impaired glucose tolerance complicated with hypertension leads to tubulointerstitial fibrosis in rat kidney. Imidazolone deposition and renin production in peritubular capillary endothelial cells may play a role in the development of tubulointerstitial fibrosis.

Original languageEnglish
Pages (from-to)656-664
Number of pages9
JournalJournal of Nephrology
Volume24
Issue number5
DOIs
Publication statusPublished - Sep 1 2011

Keywords

  • Hypertension
  • Imidazolone
  • Impaired glucose tolerance
  • Peritubular capillary endothelial cells
  • Renin
  • Tubulointerstitial fibrosis

ASJC Scopus subject areas

  • Nephrology

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