Accumulation of overproduced ferritin in the chloroplast provides protection against photoinhibition induced by low temperature in tobacco plants

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Abstract

Wild-type tobacco plants (Nicotiana tabacum L. cv. Petit Havanna line SR1) and plants transformed with full-length alfalfa ferritin cDNA with the chloroplast transit peptide under the control of a Rubisco small subunit gene promoter (C3 and C8) were cold-treated at 0 °C with continuous light (250 μmol m-2 s-1). These transgenic plants had higher chlorophyll content and higher Fv/Fm chlorophyll-a fluorescence induction parameters than wild-type plants after 2 or 3 d of cold treatment in C3 and C8 transgenic plants, respectively. Thermoluminescence studies on the high-temperature bands suggest that these plants suffered less oxidative damage in comparison to the wild-type genotype. The present experiments provide evidence that transgenic tobacco lines overexpressing alfalfa ferritin, which is accumulated in the chloroplasts, may show higher tolerance to various stress factors, generating ROS including low temperature-induced photoinhibition.

Original languageEnglish
Pages (from-to)1647-1651
Number of pages5
JournalJournal of Plant Physiology
Volume165
Issue number15
DOIs
Publication statusPublished - Oct 9 2008

Fingerprint

ferritin
photoinhibition
Chloroplasts
Ferritins
Tobacco
tobacco
chloroplasts
Temperature
Medicago sativa
Genetically Modified Plants
alfalfa
transgenic plants
temperature
Ribulose-Bisphosphate Carboxylase
chlorophyll
ribulose-bisphosphate carboxylase
Chlorophyll
Nicotiana tabacum
Complementary DNA
Fluorescence

Keywords

  • Ferritin
  • Low temperature
  • Photoinhibition
  • Stress tolerance
  • Transgenic plants

ASJC Scopus subject areas

  • Plant Science

Cite this

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title = "Accumulation of overproduced ferritin in the chloroplast provides protection against photoinhibition induced by low temperature in tobacco plants",
abstract = "Wild-type tobacco plants (Nicotiana tabacum L. cv. Petit Havanna line SR1) and plants transformed with full-length alfalfa ferritin cDNA with the chloroplast transit peptide under the control of a Rubisco small subunit gene promoter (C3 and C8) were cold-treated at 0 °C with continuous light (250 μmol m-2 s-1). These transgenic plants had higher chlorophyll content and higher Fv/Fm chlorophyll-a fluorescence induction parameters than wild-type plants after 2 or 3 d of cold treatment in C3 and C8 transgenic plants, respectively. Thermoluminescence studies on the high-temperature bands suggest that these plants suffered less oxidative damage in comparison to the wild-type genotype. The present experiments provide evidence that transgenic tobacco lines overexpressing alfalfa ferritin, which is accumulated in the chloroplasts, may show higher tolerance to various stress factors, generating ROS including low temperature-induced photoinhibition.",
keywords = "Ferritin, Low temperature, Photoinhibition, Stress tolerance, Transgenic plants",
author = "A. Heged{\"u}s and T. Janda and G. Horv{\'a}th and D. Dudits",
year = "2008",
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language = "English",
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journal = "Journal of Plant Physiology",
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TY - JOUR

T1 - Accumulation of overproduced ferritin in the chloroplast provides protection against photoinhibition induced by low temperature in tobacco plants

AU - Hegedüs, A.

AU - Janda, T.

AU - Horváth, G.

AU - Dudits, D.

PY - 2008/10/9

Y1 - 2008/10/9

N2 - Wild-type tobacco plants (Nicotiana tabacum L. cv. Petit Havanna line SR1) and plants transformed with full-length alfalfa ferritin cDNA with the chloroplast transit peptide under the control of a Rubisco small subunit gene promoter (C3 and C8) were cold-treated at 0 °C with continuous light (250 μmol m-2 s-1). These transgenic plants had higher chlorophyll content and higher Fv/Fm chlorophyll-a fluorescence induction parameters than wild-type plants after 2 or 3 d of cold treatment in C3 and C8 transgenic plants, respectively. Thermoluminescence studies on the high-temperature bands suggest that these plants suffered less oxidative damage in comparison to the wild-type genotype. The present experiments provide evidence that transgenic tobacco lines overexpressing alfalfa ferritin, which is accumulated in the chloroplasts, may show higher tolerance to various stress factors, generating ROS including low temperature-induced photoinhibition.

AB - Wild-type tobacco plants (Nicotiana tabacum L. cv. Petit Havanna line SR1) and plants transformed with full-length alfalfa ferritin cDNA with the chloroplast transit peptide under the control of a Rubisco small subunit gene promoter (C3 and C8) were cold-treated at 0 °C with continuous light (250 μmol m-2 s-1). These transgenic plants had higher chlorophyll content and higher Fv/Fm chlorophyll-a fluorescence induction parameters than wild-type plants after 2 or 3 d of cold treatment in C3 and C8 transgenic plants, respectively. Thermoluminescence studies on the high-temperature bands suggest that these plants suffered less oxidative damage in comparison to the wild-type genotype. The present experiments provide evidence that transgenic tobacco lines overexpressing alfalfa ferritin, which is accumulated in the chloroplasts, may show higher tolerance to various stress factors, generating ROS including low temperature-induced photoinhibition.

KW - Ferritin

KW - Low temperature

KW - Photoinhibition

KW - Stress tolerance

KW - Transgenic plants

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U2 - 10.1016/j.jplph.2008.05.005

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