Abundance and location of DARPP-32 in striato-tegmental circuits of domestic chicks

E. Bálint, T. Kitka, G. Zachar, Á Ádám, H. C. Hemmings, A. Csillág

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The striatum is reciprocally connected to the brainstem dopaminergic nuclei and receives a strong dopaminergic input. In the present study the spatial relation between the dopaminergic and dopaminoceptive structures of the avian medial striatum (formerly: lobus parolfactorius) was observed by confocal laser scanning microscope in the domestic chick (Gallus domesticus). We also analysed the connections in the area ventralis tegmentalis and the substantia nigra. To label the dopaminergic structures, anti-tyrosine hydroxylase was used and DARPP-32 (dopamine and cAMP regulated phosphoprotein) was a marker of dopaminoceptive elements. The tyrosine hydroxylase positive fibres formed baskets of juxtapositions around the DARPP-32 containing cells of the medial striatum. However, such baskets were also observed to juxtapose DARPP-32 immunonegative cells. In the tegmentum, DARPP-32 was observed in axons descending from the telencephalon via the ansa lenticularis. These varicose fibers innervated the ventral tegmental area and substantia nigra and were often juxtaposed to dopaminergic neurons and dendrites. Approximately 40% of the striatal projection neurons targeting the ventral tegmentum, and 60% of striatal projection neurons targeting the nigra were immunoreactive to DARPP-32, as revealed by retrograde pathway tracing with Fast Blue. Endogenous dopamine may exert a retrograde synaptic effect on the afferent striato-tegmental fibers, apart from the reported extrasynaptic action. The abundance of juxtapositions observed in the avian brainstem and medial striatum corroborates the possibility of reciprocal striato-tegmental circuits, relevant to the reinforcement of behaviour.

Original languageEnglish
Pages (from-to)27-36
Number of pages10
JournalJournal of Chemical Neuroanatomy
Volume28
Issue number1-2
DOIs
Publication statusPublished - Sep 2004

Fingerprint

Corpus Striatum
Ventral Tegmental Area
Tyrosine 3-Monooxygenase
Substantia Nigra
Brain Stem
Dopamine and cAMP-Regulated Phosphoprotein 32
Neurons
Telencephalon
Dopaminergic Neurons
Dendrites
Axons
Chickens
Dopamine
Lasers
Reinforcement (Psychology)
diamidino compound 253-50

Keywords

  • Avian brain
  • Confocal microscopy
  • Dopamine
  • Medial striatum
  • Pathway tracing
  • Substantia nigra
  • Ventral tegmental area

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

Cite this

Abundance and location of DARPP-32 in striato-tegmental circuits of domestic chicks. / Bálint, E.; Kitka, T.; Zachar, G.; Ádám, Á; Hemmings, H. C.; Csillág, A.

In: Journal of Chemical Neuroanatomy, Vol. 28, No. 1-2, 09.2004, p. 27-36.

Research output: Contribution to journalArticle

Bálint, E. ; Kitka, T. ; Zachar, G. ; Ádám, Á ; Hemmings, H. C. ; Csillág, A. / Abundance and location of DARPP-32 in striato-tegmental circuits of domestic chicks. In: Journal of Chemical Neuroanatomy. 2004 ; Vol. 28, No. 1-2. pp. 27-36.
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